Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
J Insect Sci. 2006;6:1-9. doi: 10.1673/031.006.3501.
In the present study, we cloned and sequenced the entire coding regions of two glutathione S-transferase (GST) genes encoding PxGSTs and PxGSTe from the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae), along with their respective 5' and 3' flanking regions. The PxGSTs gene was composed of four exons and three introns. Based on amino acid identity with GST genes from other insects, this gene was classified as a member of the Sigma class. The gene encoding PxGSTe had an intron in the 5' flanking region. Southern blot analysis showed that PxGSTs was a single copy gene, whereas there were homologous members of the PxGSTe gene in the P. xylostella genome. RNA gel blot analysis indicated that the expression levels of both genes changed with the developmental stage of P. xylostella.
在本研究中，我们从小菜蛾（鳞翅目：菜蛾科）中克隆并测序了编码 PxGSTs 和 PxGSTe 的两个谷胱甘肽 S-转移酶（GST）基因的全长编码区及其相应的 5'和 3'侧翼区。PxGSTs 基因由四个外显子和三个内含子组成。根据与其他昆虫 GST 基因的氨基酸同一性，该基因被归类为 Sigma 类成员。编码 PxGSTe 的基因在 5'侧翼区有一个内含子。Southern blot 分析表明，PxGSTs 是一个单拷贝基因，而 P. xylostella 基因组中存在 PxGSTe 基因的同源成员。RNA 凝胶印迹分析表明，这两个基因的表达水平随小菜蛾的发育阶段而变化。
