Ishihara Atsushi, Goto Noriko, Kikkawa Misa, Ube Naoki, Ushijima Shuji, Ueno Makoto, Ueno Kotomi, Osaki-Oka Kumiko
Faculty of Agriculture, Tottori University.
The United Graduate School of Agricultural Sciences, Tottori University.
J Pestic Sci. 2018 May 20;43(2):108-113. doi: 10.1584/jpestics.D17-094.
In view of the possibility that spent mushroom substrates (SMSs) may be used as agricultural materials to prevent crop diseases, we examined the effect of treatment with a hot water extract from the SMS of on plant resistance to pathogenic infection. The extract of SMS was sprayed onto the leaves of rice plants, followed by inoculation of the leaves with the conidia of rice blast fungus. The development of lesions was suppressed by treatment with the SMS extract. The extract markedly inhibited the germination of conidia. We purified compounds , , and , which showed inhibitory effects on conidial germination, from the SMS extract of by successive solvent extraction, column chromatography, and preparative HPLC. Spectroscopic analyses revealed that , , and were phenolic acids with two carboxyl groups in common.
鉴于废弃蘑菇培养料（SMSs）有可能用作预防作物病害的农业材料，我们研究了用SMSs热水提取物处理对植物抵抗病原菌感染的影响。将SMS提取物喷洒到水稻叶片上，随后用稻瘟病菌的分生孢子接种叶片。用SMS提取物处理可抑制病斑的发展。该提取物显著抑制分生孢子的萌发。我们通过连续溶剂萃取、柱色谱和制备型高效液相色谱从SMS提取物中纯化出对分生孢子萌发有抑制作用的化合物、和。光谱分析表明，、和是具有两个共同羧基的酚酸。
