Red Manejo Biotecnológico de Recursos (RMBR), Instituto de Ecología (A.C), Xalapa 91073, Mexico.
Red Estudios Moleculares Avanzados (REMAV), Instituto de Ecología (A.C), Xalapa 91073, Mexico.
Toxins (Basel). 2020 Jun 2;12(6):366. doi: 10.3390/toxins12060366.
The maize pathogen and their mycotoxins cause damage to plants, animals, and human health. This work aimed to evaluate the effect of crude extracts (CEs) from , , and fruiting bodies on in vitro production of biomass and mycotoxins by two strains of . Stipes and pilei were separated before extraction for and . Comparative metabolomics and dereplication of phenolic compounds were used to analyze all CEs. Mushroom CEs did not significantly inhibit the production of mycelial biomass at concentrations of 2 mg mL⁻. CEs from (stipes and pilei) and pilei inhibited the production of fumonisins B1 + B2 + B3 by 54% to 80%, whereas CE from had no effect. In contrast, CE from stipes dramatically increased the concentration of fumonisins in culture media. Fusaric acid concentration was decreased in cultures by all CEs except stipes. Differences in phenolic composition of the extracts may explain the different effects of the CE treatments on the production of mycotoxins. The opposing activities of stipes and pilei from offer an opportunity to search for active compounds to control the mycotoxin production by .
玉米病原菌及其产生的霉菌毒素会对植物、动物和人类健康造成损害。本研究旨在评估 、 、 子实体的粗提取物（CEs）对两种 菌株生物量和霉菌毒素产生的体外影响。在提取前，将菌盖和菌柄分开，用于 和 分析。采用比较代谢组学和酚类化合物去重分析所有 CEs。在 2 mg mL⁻浓度下，蘑菇 CEs 对菌丝体生物量的产生没有显著抑制作用。来自 （菌盖和菌柄）和 菌盖的 CEs 抑制了 fumonisins B1 + B2 + B3 的产生，抑制率为 54%至 80%，而来自 菌柄的 CE 则没有效果。相比之下，来自 菌盖的 CE 大大增加了培养基中 fumonisins 的浓度。除了 菌柄外，所有 CEs 都降低了 culture 中 fusaric acid 的浓度。提取物中酚类成分的差异可能解释了 CE 处理对霉菌毒素产生的不同影响。来自 的菌盖和菌柄的相反作用为寻找控制 的霉菌毒素产生的活性化合物提供了机会。
