Sasaki M S, Matsubara S
Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Nov;32(5):439-45. doi: 10.1080/09553007714551191.
Human peripheral blood lymphocytes were exposed to 300 rad gamma-rays, in the presence or absence of radical scavengers, and the change in the frequency of chromosome aberrations was analysed with attention directed to the protection by scavengers against the formation of primary damage leading to chromosome aberrations. The results showed that the damage involved in the formation of exchange-type aberrations was efficiently protected by scavengers, and about 60 per cent of them resulted from indirect action that could be abolished by alcohols. The SH-compounds afforded additional protection. The comparison of protective ability with reaction rates demonstrated that the indirect effect was due to the reaction of OH radicals possibly to DNA as a target molecule, and the involvement of H and eaq-was unlikely. In contrast to the exchange-type aberrations, terminal deletions were not significantly protected, suggesting that the damage leading to the terminal deletion differed in its nature from the leading to the exchange-type aberration.
将人类外周血淋巴细胞暴露于300拉德的γ射线中，存在或不存在自由基清除剂，分析染色体畸变频率的变化，重点关注清除剂对导致染色体畸变的原发性损伤形成的保护作用。结果表明，清除剂能有效保护交换型畸变形成过程中的损伤，其中约60%是由间接作用引起的，而酒精可消除这种间接作用。含巯基化合物提供了额外的保护。将保护能力与反应速率进行比较表明，间接效应是由于羟基自由基可能与作为靶分子的DNA发生反应，氢原子和水合电子参与的可能性不大。与交换型畸变相反，末端缺失没有得到显著保护，这表明导致末端缺失的损伤在性质上与导致交换型畸变的损伤不同。
