使用一个系统对细胞色素P450s CYP6CY22和CYP6CY13进行的研究。
Studies on cytochrome P450s CYP6CY22 and CYP6CY13 using an system.
作者信息
Hirata Koichi, Jouraku Akiya, Kuwazaki Seigo, Shimomura Hajime, Iwasa Takao
机构信息
Odawara Research Center, Nippon soda Co., Ltd., 345 Takada, Odawara, Kanagawa 250-0216, Japan.
Insect Genome Research and Engineering Unit, Division of Applied Genetics, Institute of Agrobiological Sciences, NARO, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
出版信息
J Pestic Sci. 2017 Aug 20;42(3):97-104. doi: 10.1584/jpestics.D17-006.
A field-collected clone [Kushima resistant (KR) clone] was resistant to neonicotinoid insecticides (23.8- to 394-fold). RNA-seq and next-generation sequence analyses were conducted to identify nine cytochrome P450 (CYP) genes that were significantly upregulated in the KR clone as compared with those in the insecticide-susceptible clone. P450s were transiently and efficiently expressed in S2 cell to show that CYP6CY22 (c21228) and CYP6CY13 (c21368), which were the most upregulated of the nine P450s in the KR clone, did not degrade sulfoxaflor, a new class of insecticides acting on insect nAChRs, but markedly metabolized all of the neonicotinoids tested. Hence, P450s are likely to underpin neonicotinoid resistance in other aphids as well in the future, and the P450 expression protocol established here will prompt studies on P450-medidated insecticide resistance and structural analyses of relevant metabolites.
一株田间采集的克隆体[久岛抗性（KR）克隆体]对新烟碱类杀虫剂具有抗性（抗性倍数为23.8至394倍）。进行了RNA测序和新一代测序分析，以鉴定出与杀虫剂敏感克隆体相比，在KR克隆体中显著上调的9个细胞色素P450（CYP）基因。P450在S2细胞中瞬时且高效表达，结果表明，KR克隆体中9个P450中上调程度最高的CYP6CY22（c21228）和CYP6CY13（c21368）不会降解氟啶虫胺腈（一种作用于昆虫烟碱型乙酰胆碱受体的新型杀虫剂），但能显著代谢所有测试的新烟碱类杀虫剂。因此，P450可能在未来也成为其他蚜虫对新烟碱类杀虫剂产生抗性的基础，此处建立的P450表达方案将推动对P450介导的杀虫剂抗性及相关代谢物结构分析的研究。
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