Yang Yuan-Xue, Lin Rong-Hua, Li Zhuo, Wang Ai-Yu, Xue Chao, Duan Ai-Ling, Zhao Ming, Zhang Jian-Hua
Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan, China.
Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs, Beijing, China.
Front Physiol. 2021 Jan 20;11:624287. doi: 10.3389/fphys.2020.624287. eCollection 2020.
(Koch) is an economically important pest that affects legumes in worldwide. Chemical control is still the primary efficient method for management. However, the mechanism underlying insecticide resistance in has not been elucidated. A previous study observed that piperonyl butoxide (PBO) and diethyl maleate (DEM) significantly synergized imidacloprid in field populations, indicating that cytochrome P450 (P450) and glutathione S-transferase (GST) genes may play pivotal roles in imidacloprid resistance. In this study, 38 P450 genes and 10 GST genes were identified in through transcriptomic analysis. The expression levels of these P450 and GST genes were measured in susceptible (SUS) strains of under imidacloprid treatment with LC, LC, and LC doses. The expression levels of , , , , , , and were up-regulated in the three treatments. Most of these genes belong to CYP3 and CYP4 Clans. In addition, the expression levels of all P450 and GST genes in were also measured in the Juye (JY) and Linqing (LQ) field populations. The expression levels of , , and were up-regulated in the SUS strain after imidacloprid treatment at three doses, and these genes were overexpressed in the JY population. Furthermore, the sensitivity of to imidacloprid was significantly increased after knockdown of and through RNA interference. These results may help to elucidate the mechanisms underlying of imidacloprid resistance in .
豆卫矛象甲是一种在全球范围内对豆类作物有重要经济影响的害虫。化学防治仍然是主要的有效防治方法。然而，豆卫矛象甲对杀虫剂产生抗性的潜在机制尚未阐明。先前的一项研究观察到，增效醚（PBO）和顺丁烯二酸二乙酯（DEM）可显著增强吡虫啉对豆卫矛象甲田间种群的毒力，这表明细胞色素P450（P450）和谷胱甘肽S-转移酶（GST）基因可能在吡虫啉抗性中起关键作用。在本研究中，通过转录组分析在豆卫矛象甲中鉴定出38个P450基因和10个GST基因。在用LC、LC和LC剂量的吡虫啉处理下，测定了这些P450和GST基因在豆卫矛象甲敏感（SUS）品系中的表达水平。在这三种处理中，、、、、、和的表达水平上调。这些基因大多属于CYP3和CYP4家族。此外，还测定了豆卫矛象甲中所有P450和GST基因在巨野（JY）和临清（LQ）田间种群中的表达水平。在三种剂量的吡虫啉处理后，SUS品系中、和的表达水平上调，并且这些基因在JY种群中过表达。此外，通过RNA干扰敲低和后，豆卫矛象甲对吡虫啉的敏感性显著增加。这些结果可能有助于阐明豆卫矛象甲对吡虫啉产生抗性的潜在机制。
