Expression profile changes of cytochrome P450 genes between thiamethoxam susceptible and resistant strains of Aphis gossypii Glover.

Cytochrome P450 monooxygenases represent a key detoxification mechanism in neonicotinoids resistance in Aphis gossypii Glover. Synergism analysis has indicates that P450s are involved in thiamethoxam resistance. In this study, expression changes in the transcripts of P450 genes were determined in thiamethoxam-susceptible and thiamethoxam-resistant strains. Nine P450 genes in CYP3 clade were significantly overexpressed in the resistant strain (especially CYP6CY14, which was increased 17.67-fold) compared with the susceptible strain. Transcripts of ecdysone synthesis-related P450 genes, including CYP302A1, CYP306A1, CYP307A1 and CYP315A1, were up-regulated in the resistant strain, which may accelerate molting hormone production. The ecdysone response genes (ecdysone receptor (EcR), ultra-spiracle (USP) and Broad-complex protein (Br-C)) were overexpressed in the resistant strain. RNA interference (RNAi) targeting CYP6CY14 significantly increased the sensitivity of the resistant aphid to thiamethoxam. The results of the present study indicate the possible involvement of these P450 genes in thiamethoxam resistance. Our findings may facilitate further work to validate the roles of these P450s in thiamethoxam resistance based on heterologous expression, and show that screening the expression changes in P450 genes can reveal the impact of thiamethoxam on ecdysone synthesis-related P450 genes. These results are useful for understanding the mechanism of thiamethoxam resistance and will contribute to the management of insecticide-resistant cotton aphids in China.