Song Ping, Yuan Kai, Ji Xiao-Jun, Ren Lu-Jing, Zhang Sen, Wen Jian-Ping, Huang He
School of Chemical Engineering and Technology, Department of Biochemical Engineering, Tianjin University, Tianjin, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
Front Microbiol. 2018 Oct 10;9:2352. doi: 10.3389/fmicb.2018.02352. eCollection 2018.
Pneumocandin B is an important antifungal drug precursor produced by filamentous fungus . The high broth viscosity of cultures of this organism results in lower oxygen solubility and higher energy consumption for agitation and aeration, which mostly caused by the morphologies of filamentous fungi in submerged culture. In this study, the effects of different seed medium nitrogen sources on morphology and fermentation behavior of were investigated, and cotton seed powder resulted in small, compact pellets. Moreover, pneumocandin B yield in Erlenmeyer flasks were increased by 22.9%. Furthermore, pneumocandin B yield in a 50-L fermenter reached 2,100 mg/L and the dissolved oxygen maintained above 30%. Additionally, activities of phosphofructokinase (PFK), isocitrate dehydrogenase (ICDH), glucose 6-phosphate dehydrogenase (G6PDH), and malic enzyme (ME) were increased by 87.5, 50, 41.6, and 10.7%, respectively. This study demonstrates the feasibility and advantages of using cotton seed powder for controlling the fungal morphology and improving the product yield in pneumocandin fermentations.
pneumocandin B是丝状真菌产生的一种重要抗真菌药物前体。该微生物培养物的高发酵液粘度导致较低的氧溶解度以及搅拌和通气的较高能量消耗，这主要是由丝状真菌在深层培养中的形态所致。在本研究中，研究了不同种子培养基氮源对其形态和发酵行为的影响，棉籽粉产生了小而紧密的菌球。此外，锥形瓶中pneumocandin B的产量提高了22.9%。此外，50-L发酵罐中pneumocandin B的产量达到2100 mg/L，溶解氧维持在30%以上。此外，磷酸果糖激酶（PFK）、异柠檬酸脱氢酶（ICDH）、葡萄糖6-磷酸脱氢酶（G6PDH）和苹果酸酶（ME）的活性分别提高了87.5%、50%、41.6%和10.7%。本研究证明了在pneumocandin发酵中使用棉籽粉控制真菌形态和提高产品产量的可行性和优势。
