Yang Wenjun, Ruan Lifang, Tao Jiangming, Peng Donghai, Zheng Jinshui, Sun Ming
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
College of Informatics, Huazhong Agricultural University, Wuhan, China.
Front Microbiol. 2018 Oct 11;9:2242. doi: 10.3389/fmicb.2018.02242. eCollection 2018.
formulation losing its activity under field conditions due to UV radiation and photoprotection of based on melanin has attracted the attention of researchers for many years. Here, a single amino acid substitution (G272E) in homogentisate 1,2-dioxygenase was found to be responsible for pigment overproduction in BMB181, a derivative of BMB171. Disrupting the gene encoding homogentisate dioxygenase in BMB171 induced the accumulation of the homogentisic acid and provoked an increased pigment formation. To gain insights into homogentisate 1,2-dioxygenase in , we constructed a total of 14 mutations with a single amino acid substitution, and six of the mutant proteins were found to affect the melanin production when substituted by alanine. This study provides a new way to construct pigment-overproducing strains by impairing the homogentisate dioxygenase with a single mutation in , and the findings will facilitate a better understanding of this enzyme.
由于紫外线辐射，制剂在田间条件下失去活性，基于黑色素的光保护作用多年来一直吸引着研究人员的关注。在此，发现尿黑酸1,2-双加氧酶中的单个氨基酸取代（G272E）是BMB171的衍生物BMB181中色素过度产生的原因。破坏BMB171中编码尿黑酸双加氧酶的基因会诱导尿黑酸的积累并引发色素形成增加。为了深入了解尿黑酸1,2-双加氧酶，我们构建了总共14个单氨基酸取代的突变体，其中6个突变蛋白在被丙氨酸取代时被发现影响黑色素的产生。本研究提供了一种通过在尿黑酸双加氧酶中进行单个突变来构建色素高产菌株的新方法，这些发现将有助于更好地理解这种酶。
