Wei Banghong, Yang Zhigang, Cheng Yongxu, Zhou Junyu, Yang Hang, Zhang Long, Yang Xiaozhen
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China.
Centre for Research on Environmental Ecology and Fish Nutrition, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China.
Front Physiol. 2018 Oct 10;9:1430. doi: 10.3389/fphys.2018.01430. eCollection 2018.
As representatives of n-6 and n-3 fatty acids, many studies have analyzed the use of soybean oil and linseed oil rich in linoleic acid (18:2n-6, LA) and α-linolenic acid (18:3n-3, LNA) as better substitutes for fish oil. In aquatic animals, different dietary ratios of LA and LNA could have significant effects on growth, lipid metabolism, immune response, and reproduction. To assess the nutritive value of these two fatty acids in Chinese mitten crab (), we performed transcriptome analysis and label-free quantification proteomic analysis of the hepatopancreas from mitten crabs fed with LA or LNA diet. Parallel reaction monitoring was used to confirm the reliability of the proteomic analysis. A total of 186 proteins were differentially expressed with fold change ≥1.5 or ≤0.666. Among the 186 proteins, 116 were upregulated and 70 were downregulated in the LA than LNA. Most of these proteins participate in cellular process and metabolism process and have molecular functions such as binding and catalytic activity; the cellular component of these proteins are cell, cell part, membrane, and membrane part. A total of 18 proteins were identified to be related to lipid, carbohydrate, and protein metabolism, and they mainly participate in digestive enzyme activities, fatty acid transport, and glycolysis. Our results provide new insights for further investigation into the replacement of fish oil from mitten crabs with vegetable oils and enable us to better understand the different roles and nutrition value of LA and LNA in mitten crabs.
作为n-6和n-3脂肪酸的代表，许多研究分析了富含亚油酸（18:2n-6，LA）和α-亚麻酸（18:3n-3，LNA）的大豆油和亚麻籽油作为鱼油更好替代品的用途。在水生动物中，不同的LA和LNA日粮比例可能对生长、脂质代谢、免疫反应和繁殖产生显著影响。为了评估这两种脂肪酸在中国绒螯蟹中的营养价值，我们对喂食LA或LNA日粮的绒螯蟹肝胰腺进行了转录组分析和无标记定量蛋白质组分析。采用平行反应监测来确认蛋白质组分析的可靠性。共有186种蛋白质差异表达，变化倍数≥1.5或≤0.666。在这186种蛋白质中，与LNA相比，LA中有116种上调，70种下调。这些蛋白质大多参与细胞过程和代谢过程，具有结合和催化活性等分子功能；这些蛋白质的细胞成分是细胞、细胞部分、膜和膜部分。共有18种蛋白质被鉴定与脂质、碳水化合物和蛋白质代谢相关，它们主要参与消化酶活性、脂肪酸转运和糖酵解。我们的结果为进一步研究用植物油替代绒螯蟹中的鱼油提供了新的见解，并使我们能够更好地了解LA和LNA在绒螯蟹中的不同作用和营养价值。
