Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
Int J Mol Med. 2019 Jan;43(1):177-184. doi: 10.3892/ijmm.2018.3953. Epub 2018 Oct 24.
Adipose‑derived stem cells (ADSCs) have an immunomodulatory role in vascularized composite tissue allo‑transplantation (VCA). However, the specific effects of ADSCs on lymphocytes remain to be fully elucidated. The present study examined the changes in T cells co‑cultured with ADSCs in terms of the proliferation by Cell Counting Kit‑8 assay, cell cycle profile and apoptosis by flow cytometry, inflammatory cytokine production by polymerase chain reaction and ELISA, in addition to the expression of survival proteins by western blotting. The ADSCs reduced the viability of Jurkat T cells and downregulated the transcription of tumor necrosis factor‑α and transforming growth factor‑β1. Co‑culture with ADSCs also induced apoptosis and increased the levels of phosphorylated c‑Jun N‑terminal kinase in the T cells. Taken together, these findings confirmed that ADSCs modulate the host immune response by suppressing T cells.
脂肪来源的干细胞（ADSCs）在血管化复合组织同种异体移植（VCA）中具有免疫调节作用。然而，ADSCs 对淋巴细胞的具体作用仍有待充分阐明。本研究通过 Cell Counting Kit-8 检测、流式细胞术检测细胞周期和凋亡、聚合酶链反应和 ELISA 检测炎症细胞因子的产生以及 Western blot 检测存活蛋白的表达，检测了与 ADSCs 共培养的 T 细胞的增殖变化。ADSCs 降低了 Jurkat T 细胞的活力，并下调了肿瘤坏死因子-α和转化生长因子-β1 的转录。与 ADSCs 共培养还诱导了 T 细胞的凋亡，并增加了磷酸化 c-Jun N-末端激酶的水平。综上所述，这些发现证实 ADSCs 通过抑制 T 细胞来调节宿主免疫反应。
