Graduate School, Tianjin Medical University, Tianjin 300070, P.R. China.
Department of Neurosurgery, Logistics University of Chinese People's Armed Police Force, Tianjin 300162, P.R. China.
Mol Med Rep. 2018 Dec;18(6):5502-5510. doi: 10.3892/mmr.2018.9570. Epub 2018 Oct 22.
In the present study, the microRNA (miRNA) expression profiles of rats exposed to high altitude hypoxia and normal conditions were obtained from miRNA array analysis. Bioinformatics analyses, including the use of the Gene Oncology and Kyoto Encyclopedia of Genes and Genomes databases, were used to identify the genes and pathways, which were specifically associated with high altitude hypoxic environment‑associated miRNAs. A total of 26 miRNAs were differentially expressed in the two groups, comprising six upregulated and 20 downregulated miRNAs. In the present study, a novel pattern of upregulated miRNAs and their associated pathways were constructed, including proteoglycans in cancer, spliceosome, gluamatergic synapse, glycolysis/gluconeogenesis, Foxo, cGMP‑PKG and p53 signaling pathways, which may provide novel targets for diagnosing and understanding the mechanism of high altitude hypoxia‑induced disease.
在本研究中，通过 miRNA 芯片分析获得了暴露于高原缺氧和正常条件下的大鼠的 miRNA 表达谱。使用基因肿瘤学和京都基因与基因组百科全书数据库等生物信息学分析方法，鉴定了与高原缺氧环境相关 miRNA 特异性相关的基因和途径。在这两组中，共有 26 个 miRNA 表达差异，包括 6 个上调和 20 个下调 miRNA。在本研究中，构建了一个新型的上调 miRNA 及其相关途径模式，包括癌症中的蛋白聚糖、剪接体、谷氨酸能突触、糖酵解/糖异生、Foxo、cGMP-PKG 和 p53 信号通路，这可能为诊断和理解高原缺氧诱导疾病的机制提供新的靶点。
