Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, Université de La Réunion, Saint-Denis, France.
Département Hygiène Sécurité Environnement (HSE), IUT La Réunion, Université de La Réunion, Saint-Pierre, France.
Biotechnol Prog. 2019 Jan;35(1):e2738. doi: 10.1002/btpr.2738. Epub 2018 Nov 21.
Filamentous fungi have gained growing interest as sources of diverse pigmented secondary metabolites. Some specific polyketides from Ascomycetous species have demonstrated a wide range of industrial applications in food, cosmetic, textile, and in the design of pharmaceutical products. The formulation of recipes containing fungal polyketides has increased over recent years. Fusarium strains were proven useful to mankind in a variety of technologies. Nevertheless, there is still need of new isolates of Fusarium for use in emerging and already existing fields. In this article, we report the concomitant production of the bioactive red bikaverin along with two novel purple pigments by the phytopathogenic Fusarium oxysporum LCP531 strain isolated from soil. In literature, the production of purple pigment had only been described in cultures of Fusarium Fujikuroi, Fusarium verticillioides, and Fusarium graminearum. The production of these naphthoquinonic pigments, their distribution (either produced in mycelia or excreted in liquid medium) and their chemical profiles were investigated with respect to nutrient composition. The pigments were extracted by using a pressurized liquid extraction method, monitored by colorimetric analysis and characterized by HPLC-DAD chromatography. To our knowledge, this is the first report of these two novel wild-type purple naphtoquinones pigments along with bikaverin, where additionally, the culture conditions were put into perspective to optimize fermentation cultures and extraction process accordingly to the pigment/biomolecule desired. These colored naphthoquinones should be promising fungal functional compounds which could be expected to have a place of choice, along with other antibacterial, antifungal, antiviral, anticancer, and antineoplastic derivatives. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2738, 2019.
丝状真菌作为各种色素次生代谢物的来源越来越受到关注。一些特定的聚酮类物质来自子囊菌物种，在食品、化妆品、纺织品和药物产品设计方面具有广泛的工业应用。近年来，含有真菌聚酮类物质的配方有所增加。镰刀菌菌株在多种技术中对人类有用。然而，在新兴和现有领域仍然需要新的镰刀菌分离株。在本文中，我们报道了植物病原菌尖孢镰刀菌 LCP531 菌株同时产生生物活性红比卡宁和两种新型紫色色素，该菌株从土壤中分离得到。在文献中，紫色色素的产生仅在尖孢镰刀菌、串珠镰刀菌和禾谷镰刀菌的培养物中被描述过。这些萘醌类色素的产生、分布（在菌丝中产生或在液体培养基中分泌）及其化学特征是根据营养成分进行研究的。使用加压液体萃取法提取色素，通过比色分析进行监测，并通过 HPLC-DAD 色谱进行表征。据我们所知，这是首次报道这两种新型野生型紫色萘醌类色素与比卡宁一起产生，此外，还根据所需的色素/生物分子对培养条件进行了优化，以相应地优化发酵培养和提取工艺。这些有色萘醌类物质应该是很有前途的真菌功能化合物，有望与其他具有抗菌、抗真菌、抗病毒、抗癌和抗肿瘤作用的衍生物一起占据一席之地。 © 2018 美国化学工程师协会生物技术进展，35:e2738，2019 年。
