大规模基因组分析揭示了人类肠道病毒中反复出现的种间重组模式。

Large-scale genomic analysis reveals recurrent patterns of intertypic recombination in human enteroviruses.

机构信息

Bioinformatics Laboratory, Department of Biochemistry and Biotechnology, University of Thessaly, Larisa 41500, Greece.

Molecular Virology Laboratory, Department of Biochemistry and Biotechnology, University of Thessaly, Larisa 41500, Greece; Microbiology Laboratory, Department of Medical Laboratories, University of Applied Sciences of Thessaly, Larisa 41110, Greece.

出版信息

Virology. 2019 Jan 2;526:72-80. doi: 10.1016/j.virol.2018.10.006. Epub 2018 Oct 23.

DOI:10.1016/j.virol.2018.10.006

PMID:30366300

Abstract

Recombination is a driving force for the emergence, evolution and virulence/epidemics of viruses, comprising the Enterovirus genus of the Picornaviridae family, important for human and animal health. By analyzing 2949 complete genomes/coding sequences, we provide a thorough and up-to-date overview of the genome-wide patterns and hotspots of intertypic recombination between the genogroups of this genus. Two prominent recombination hotspots are identified/verified, at the 5'UTR-capsid region junction, and at the beginning of the P2 region. In general, P2 was enriched in recombination events. Key phylogenetic groups implicated in recombination events are E71 and CVA6 in Enterovirus A species, E30 and E6 in Enterovirus B species, polioviruses 1 and 2 in Enterovirus C species. In addition, many events involve recombination partners that have not been sequenced yet, thus strongly suggesting a large environmental reservoir of genetic variation with a high potential for the emergence of new modified pathogens by recombination.

摘要

重组是病毒出现、进化和毒力/流行的驱动力，包括小核糖核酸病毒科肠道病毒属，对人类和动物健康很重要。通过分析 2949 个完整基因组/编码序列，我们提供了该属各基因群之间全基因组范围的重组模式和热点的全面和最新概述。在 5'UTR-衣壳区域交界处和 P2 区域起始处鉴定/验证了两个突出的重组热点。一般来说，P2 富含重组事件。涉及重组事件的关键系统发育群是肠道病毒 A 种的 E71 和 CVA6、肠道病毒 B 种的 E30 和 E6、肠道病毒 C 种的脊髓灰质炎病毒 1 和 2。此外，许多事件涉及尚未测序的重组伙伴，因此强烈表明存在具有高潜力的遗传变异环境库，通过重组可能会出现新的改良病原体。