Sosnovtseva Anastasiia O, Le Thi Hoa, Karpov Dmitry S, Vorobyev Pavel O, Gumennaya Yana D, Alekseeva Olga N, Chumakov Peter M, Lipatova Anastasia V
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Int J Mol Sci. 2025 Jan 22;26(3):923. doi: 10.3390/ijms26030923.
Non-pathogenic natural and recombinant strains of human are the subject of ongoing study with some strains having been approved for use as anticancer agents. The efficacy of oncolytic virotherapy depends upon identifying the receptor utilized by a specific strain for cell entry, and the presence of this receptor on the surface of cancer cells. Accordingly, a rapid and straightforward approach to determining the enteroviral receptors is necessary for developing an effective patient-specific, virus-based cancer therapy. To this end, we created a panel of seven lines with double knockouts on the background of the HEK293T cell line, which lacks the gene. In these lines, the main viral receptor genes, including , , , , , , and , were knocked out using the CRISPR/Cas9 system. The panel of lines was validated on twelve different types, providing a basis for studying the molecular mechanisms of enterovirus entry into cells, and for developing new therapeutic strains.
非致病性天然和重组人类毒株是正在进行的研究主题，其中一些毒株已被批准用作抗癌剂。溶瘤病毒疗法的疗效取决于确定特定毒株用于进入细胞的受体，以及癌细胞表面该受体的存在情况。因此，开发有效的患者特异性基于病毒的癌症疗法需要一种快速且直接的方法来确定肠道病毒受体。为此，我们在缺乏该基因的HEK293T细胞系背景下创建了一组七个双敲除细胞系。在这些细胞系中，使用CRISPR/Cas9系统敲除了主要的病毒受体基因，包括、、、、、和。该细胞系组在十二种不同类型上进行了验证，为研究肠道病毒进入细胞的分子机制以及开发新的治疗毒株提供了基础。
