School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China.
School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China.
J Chromatogr A. 2018 Dec 7;1579:115-120. doi: 10.1016/j.chroma.2018.10.024. Epub 2018 Oct 15.
A fast and facile method was developed for on-site detection of aflatoxins (AFs) in moldy agricultural products using thin layer chromatography combined with surface-enhanced Raman spectroscopy (TLC-SERS). Four different AFs were successfully separated by TLC and then a small portable Raman spectrometer, with gold colloids as the SERS-active substrate, was applied to identify the separated spots. TLC-SERS application to on-site detection of AFs was systematically investigated. Qualitative and quantitative AF detection was found to be easily accomplished and limits of detection were estimated to be 1.5 × 10, 1.1 × 10, 1.2 × 10, and 6.0 × 10 M for AFB, AFB, AFG, and AFG, respectively. The proposed method was also highly selective, enabling successful AF identifications in complex extracts from moldy peanuts. The study showed that TLC-SERS could be effectively used for separation and detection of these four AFs, demonstrating good prospects for on-site qualitative screening of agricultural products.
建立了一种快速简便的方法，用于使用薄层色谱结合表面增强拉曼光谱（TLC-SERS）现场检测霉变农产品中的黄曲霉毒素（AFs）。通过 TLC 成功分离了四种不同的 AF，然后使用带有金胶体的小型便携式拉曼光谱仪作为 SERS 活性基底来识别分离的斑点。对 TLC-SERS 用于现场检测 AF 进行了系统研究。结果发现，定性和定量检测 AF 很容易完成，检测限估计分别为 1.5×10、1.1×10、1.2×10 和 6.0×10 M 用于 AFB、AFB、AFG 和 AFG。该方法还具有高度选择性，能够成功识别霉变花生中复杂提取物中的 AF。研究表明，TLC-SERS 可有效用于这四种 AF 的分离和检测，为农产品现场定性筛选展示了良好的前景。
