University of Milano Bicocca, Materials Science Department, via R. Cozzi 55, I-20125, Milan, Italy.
University of Milano Bicocca, Materials Science Department, via R. Cozzi 55, I-20125, Milan, Italy; University of Milano Bicocca, COMiB, via R. Cozzi 55, I-20125, Milan, Italy.
Cont Lens Anterior Eye. 2019 Apr;42(2):212-215. doi: 10.1016/j.clae.2018.10.004. Epub 2018 Oct 23.
The purpose was to evaluate if there are differences between tears of contact lens (CL) wearers of different materials detectable by measuring the hydrodynamic diameter of tear components through photon correlation spectroscopy (PCS).
Tears of 59 CL wearers and tears of 39 non-wearers were collected by glass capillary. Wearers were divided into groups depending on the CL material: (i) hydrogels of II FDA group (H-II, 15 subjects), (ii) hydrogels of IV FDA group (H-IV, 13 subjects), (iii) silicone hydrogels (SH, 31 subjects). PCS analyses were performed at 25 °C on samples diluted with deionized water with tear concentration (10 ± 2)% / to obtain, for each subject, the average hydrodynamic diameter (d) of tear components by analyzing intensity fluctuations in time of scattered light.
Means of d calculated on each group were found, on increasing order, to be 256 nm (std dev 18 nm) for non-wearers, 297 nm (std dev 45 nm) for H-II, 360 nm (std dev 76 nm) for SH, and 391 nm (std dev 85 nm) for H-IV with statistical differences between each group of wearers compared to non-wearers and between groups of wearers except between SH and H-IV.
PCS reveals the differences between tears of CL wearers of different materials, not only between tears of wearers and non-wearers.
通过光子相关光谱（PCS）测量泪液成分的流体动力直径，评估不同材料的隐形眼镜（CL）佩戴者的泪液是否存在差异。
使用玻璃毛细管收集 59 名 CL 佩戴者和 39 名非佩戴者的泪液。根据 CL 材料将佩戴者分为以下几组：（i）II 类 FDA 组水凝胶（H-II，15 名受试者），（ii）IV 类 FDA 组水凝胶（H-IV，13 名受试者），（iii）硅水凝胶（SH，31 名受试者）。在 25°C 下，用去离子水稀释样品（泪液浓度为 10±2）%/，以获得每个受试者泪液成分的平均流体动力直径（d），通过分析散射光强度随时间的波动。
在每个组中计算的 d 的平均值按增加的顺序分别为：非佩戴者为 256nm（标准差 18nm），H-II 为 297nm（标准差 45nm），SH 为 360nm（标准差 76nm），H-IV 为 391nm（标准差 85nm），与非佩戴者相比，佩戴者的每组与非佩戴者之间以及佩戴者组之间（SH 和 H-IV 之间除外）均存在统计学差异。
PCS 揭示了不同材料的 CL 佩戴者的泪液之间的差异，不仅在佩戴者和非佩戴者之间。
