Aix Marseille Univ, IRD, MEPHI, IHU Méditerranée Infection, Marseille, France.
Centre d'Études et de Recherche de Djibouti (CERD), Institut de Recherche Médicinale (IRM), Djibouti, Djibouti.
Sci Rep. 2018 Oct 26;8(1):15341. doi: 10.1038/s41598-018-33737-9.
In low-income countries of the Horn of Africa, pulmonary infections are usually considered as tuberculosis, which diagnosis relies on clinical data and positive microscopic observation. This strategy allows non-tuberculous mycobacteria to escape detection, facilitating their emergence in populations. A non-tuberculous mycobacterium strain FB-527 was unexpectedly cultured from the sputum of a Djiboutian patient otherwise diagnosed with multi-drug resistant (MDR) tuberculosis. The sequencing of the rpoB and 16S rRNA genes showed that the isolate was identical to strain FI-09026 previously named "Mycobacterium simulans" and reported only once from a Somali patient. Strain FB-527 mimicked Mycobacterium tuberculosis colonies and enzymatic profile using API ZYM strip and was in vitro resistant to rifampicin and isoniazid. Isolation of two MDR mycobacteria complicated the diagnosis and therapeutic management of the patient. We here report on the complete description of strain FB-527 and strain FI-09026 including genome sequencing, finalizing the description of the proposed new species "Mycobacterium simulans".
在非洲之角的低收入国家，肺部感染通常被认为是结核病，其诊断依赖于临床数据和显微镜下的阳性观察。这种策略使得非结核分枝杆菌得以逃避检测，促进了它们在人群中的出现。从一名被诊断为耐多药（MDR）结核病的吉布提患者的痰液中意外培养出一株非结核分枝杆菌 FB-527 菌株。rpoB 和 16S rRNA 基因的测序表明，该分离株与之前命名为“模拟分枝杆菌”的 FI-09026 菌株完全相同，仅从一名索马里患者中报告过一次。FB-527 菌株模仿结核分枝杆菌菌落和酶谱，使用 API ZYM 条带，并在体外对利福平利福平和异烟肼具有耐药性。两种 MDR 分枝杆菌的分离使患者的诊断和治疗管理复杂化。我们在此报告了 FB-527 菌株和 FI-09026 菌株的完整描述，包括基因组测序，最终完成了拟议的新物种“模拟分枝杆菌”的描述。
