蛋白工程化大面积脂肪来源干细胞片用于创面愈合。
Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing.
机构信息
Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.
Institute for Human Tissue Restoration, Department of Plastic & Reconstructive Surgery, Yonsei University College of Medicine, Seoul, South Korea.
出版信息
Sci Rep. 2018 Oct 26;8(1):15869. doi: 10.1038/s41598-018-34119-x.
Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db/db mice in vivo, to accelerate 30% faster wound closure with a high closure effect (ε) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.
人脂肪来源的干细胞（hADSCs）通过工程化可溶性细胞黏附分子（CAMs）形成了坚固的细胞片层，这使得大规模收获 hADSC 片层的独特方法成为可能。作为一种可溶性 CAM，纤连蛋白（FN）（100 pg/ml）可以增强细胞的增殖速度，并控制细胞间和细胞与基底的相互作用。通过这种 FN 的工程化，可以通过光热法获得可转移的 hADSC 片层，该片层是一个自由站立的片层（122.6mm）。在光热法收获 hADSC 片层的过程中，细胞间的胶原层和导电聚合物膜（CP）解离，以保护细胞免受近红外（NIR）源的直接暴露。hADSC 片层被应用于遗传性糖尿病 db/db 小鼠的慢性伤口中，以加快 30%的愈合速度，愈合效果（ε）比对照组高。这些结果表明，CAM 和胶原的工程化允许 hADSC 片层的收获，这可以扩展到工程化各种干细胞片层，以实现高效治疗。
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