利用荧光激活细胞分选技术（FACS）从人肌肉中纯化肌源性祖细胞。
Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS).
作者信息
Pakula Anna, Spinazzola Janelle M, Gussoni Emanuela
机构信息
Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA.
Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
出版信息
Methods Mol Biol. 2019;1889:1-15. doi: 10.1007/978-1-4939-8897-6_1.
Abstract
Primary myoblasts derived from human tissue are a valuable tool in research of muscle disease and pathophysiology. However, skeletal muscle biopsies, especially from diseased muscle, contain a plethora of non-myogenic cells, necessitating purification of the myogenic cell population. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.
摘要
源自人体组织的原代成肌细胞是肌肉疾病和病理生理学研究中的宝贵工具。然而，骨骼肌活检样本，尤其是来自患病肌肉的样本，包含大量非成肌细胞，因此需要对成肌细胞群体进行纯化。本方案描述了从人体骨骼肌活检样本中解离细胞并通过荧光激活细胞分选（FACS）富集高纯度成肌细胞群体的技术。我们还描述了评估成肌性和群体扩增以便后续进行体外研究的方法。
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