利用CRISPR/Cas9介导的MyoD靶向实现肌肉卫星细胞向脂肪细胞的转分化。
Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD.
作者信息
Chen Jingjuan, Wang Chao, Kuang Shihuan
机构信息
Department of Animal Science, Purdue University, West Lafayette, IN, USA.
Center for Cancer Research, Purdue University, West Lafayette, IN, USA.
出版信息
Methods Mol Biol. 2019;1889:25-41. doi: 10.1007/978-1-4939-8897-6_3.
Brown adipocytes dissipate energy through non-shivering thermogenesis mediated by UCP1 protein, hence representing a powerful target to overcome obesity due to energy surplus. However, brown adipocytes are scarce in adult humans, especially in obese subjects, urging the development of novel strategies to boost the number of these thermogenic adipocytes from a therapeutical perspective. In this regard, transdifferentiation of myoblasts into brown adipocytes represents a promising approach. Here, we describe a method that we have recently developed to transdifferentiate myoblasts into brown adipocytes through CRISPR/Cas9-medidated targeting of MyoD, the master myogenic regulatory factor.
棕色脂肪细胞通过由解偶联蛋白1（UCP1）介导的非颤抖性产热来消耗能量，因此是克服因能量过剩导致肥胖的有力靶点。然而，棕色脂肪细胞在成年人体内稀少，尤其是在肥胖个体中，这促使人们从治疗角度开发新策略来增加这些产热脂肪细胞的数量。在这方面，将成肌细胞转分化为棕色脂肪细胞是一种有前景的方法。在此，我们描述了一种我们最近开发的方法，即通过CRISPR/Cas9介导的对成肌主调节因子MyoD的靶向作用，将成肌细胞转分化为棕色脂肪细胞。
Zotero 插件