Mehta Frea, Theunissen Ruud, Post Mark J
Department of Physiology, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands.
Methods Mol Biol. 2019;1889:111-125. doi: 10.1007/978-1-4939-8897-6_8.
Cultured meat, or tissue engineered meat, is a promising alternative to conventional meat production. In order to realistically mimic the multiple tissue types found in beef, food-compatible methods for bovine fat tissue engineering must be developed. We present a protocol for the isolation of adipose tissue-derived preadipocytes and subsequent adipogenic differentiation through free fatty acid stimulation. Differentiating preadipocytes can be either grown in 2D culture conditions or seeded in 3D alginate scaffolds. Differentiation is visually confirmed through lipophilic staining.
培养肉，即组织工程肉，是传统肉类生产的一种有前景的替代方案。为了切实模拟牛肉中发现的多种组织类型，必须开发与食品兼容的牛脂肪组织工程方法。我们提出了一种通过游离脂肪酸刺激分离脂肪组织来源的前脂肪细胞并随后进行脂肪生成分化的方案。分化的前脂肪细胞既可以在二维培养条件下生长，也可以接种到三维海藻酸盐支架中。通过亲脂性染色在视觉上确认分化情况。
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