Lee Mi-Jeong, Fried Susan K
Division of Endocrinology, Diabetes and Nutrition, Boston University School of Medicine, Boston, Massachusetts, USA.
Methods Enzymol. 2014;538:49-65. doi: 10.1016/B978-0-12-800280-3.00004-9.
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be isolated from distinct adipose depots and cultured in vitro, have clear translational relevance. We describe the methods to isolate, culture, and differentiate human ASCs to adipocytes that respond to physiologically relevant hormones, such as insulin and β-adrenergic agonists. We also describe methods for assaying hormonal effects on glucose transport and lipolysis.
肥胖正呈流行趋势，因此人们对调节脂肪组织发育和功能的机制越来越感兴趣。尽管小鼠脂肪细胞系对许多机制研究有用，但可从不同脂肪库分离并在体外培养的原代人脂肪基质细胞（ASC）具有明确的转化相关性。我们描述了分离、培养人ASC并将其分化为对生理相关激素（如胰岛素和β-肾上腺素能激动剂）有反应的脂肪细胞的方法。我们还描述了检测激素对葡萄糖转运和脂解作用的方法。
