通过液相色谱-串联质谱分析制备用于自下而上蛋白质组学的增殖牛原代骨骼肌细胞
Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis.
作者信息
Veiseth-Kent Eva, Høst Vibeke, Pedersen Mona E
机构信息
Nofima AS, Osloveien 1, NO-1433, Aas, Norway.
出版信息
Methods Mol Biol. 2019;1889:255-266. doi: 10.1007/978-1-4939-8897-6_15.
This chapter outlines a method for sample preparation for bottom-up proteomics by LC-MSMS analysis of in vitro proliferated bovine primary skeletal muscle cells. The protocol describes the isolation of bovine primary skeletal muscle cells, extraction of proteins, proteolytic digestion of proteins, and desalting of the final peptide samples. The final peptide samples can be analyzed using various LC-MSMS systems after reconstitution in a suitable elution buffer.
本章概述了一种通过液相色谱-串联质谱（LC-MSMS）分析体外增殖的牛原代骨骼肌细胞进行自下而上蛋白质组学样品制备的方法。该方案描述了牛原代骨骼肌细胞的分离、蛋白质提取、蛋白质的蛋白酶解消化以及最终肽样品的脱盐。最终的肽样品在合适的洗脱缓冲液中复溶后，可使用各种LC-MSMS系统进行分析。
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