Bryantsev Anton L, Castillo Lizzet, Oas Sandy T, Chechenova Maria B, Dohn Tracy E, Lovato TyAnna L
Department of Molecular and Cellular Biology, Kennesaw State University, Kennesaw, GA, USA.
Department of Biology, University of New Mexico, Albuquerque, NM, USA.
Methods Mol Biol. 2019;1889:267-281. doi: 10.1007/978-1-4939-8897-6_16.
Drosophila is a useful model organism for studying the molecular signatures that define specific muscle types during myogenesis. It possesses significant genetic conservation with humans for muscle disease causing genes and a lack of redundancy that simplifies functional analysis. Traditional molecular methods can be utilized to understand muscle developmental processes such as Western blots, in situ hybridizations, RT-PCR and RNAseq, to name a few. However, one challenge for these molecular methods is the ability to dissect different muscle types. In this protocol we describe some useful techniques for extracting muscles from the pupal and adult stages of development using flight and jump muscles as an example.
果蝇是一种有用的模式生物，用于研究在肌生成过程中定义特定肌肉类型的分子特征。它在导致肌肉疾病的基因方面与人类具有显著的遗传保守性，并且缺乏冗余性，这简化了功能分析。传统的分子方法可用于理解肌肉发育过程，如蛋白质免疫印迹、原位杂交、逆转录聚合酶链反应和RNA测序等。然而，这些分子方法面临的一个挑战是能够剖析不同的肌肉类型。在本方案中，我们以飞行和跳跃肌肉为例，描述了一些从发育中的蛹期和成虫期提取肌肉的有用技术。
