枯草芽孢杆菌中碱性磷酸酶和磷酸二酯酶调控基因phoP的克隆及核苷酸序列
Cloning and nucleotide sequence of phoP, the regulatory gene for alkaline phosphatase and phosphodiesterase in Bacillus subtilis.
作者信息
Seki T, Yoshikawa H, Takahashi H, Saito H
出版信息
J Bacteriol. 1987 Jul;169(7):2913-6. doi: 10.1128/jb.169.7.2913-2916.1987.
Abstract
Two DNA fragments which complement the alkaline phosphatase-negative mutation phoP of Bacillus subtilis were cloned from a B. subtilis chromosome with the prophage vector phi CM (a derivative of phi 105). One of the fragments contained the regulatory gene phoR in addition to phoP. Nucleotide sequence analysis of the phoP region revealed that the phoP gene product consists of 241-amino-acid residues and that the sequence of these amino acids is extensively homologous with the sequence of the phoB gene product. This protein is the positive regulator for the phosphate regulon in Escherichia coli. It therefore appears that phoP is a regulatory gene for alkaline phosphatase synthesis in B. subtilis.
摘要
利用原噬菌体载体φCM（φ105的衍生物）从枯草芽孢杆菌染色体中克隆出了两个与枯草芽孢杆菌碱性磷酸酶阴性突变体phoP互补的DNA片段。其中一个片段除了含有phoP外，还包含调控基因phoR。phoP区域的核苷酸序列分析表明，phoP基因产物由241个氨基酸残基组成，并且这些氨基酸的序列与phoB基因产物的序列广泛同源。这种蛋白质是大肠杆菌中磷酸盐调节子的正调控因子。因此，phoP似乎是枯草芽孢杆菌中碱性磷酸酶合成的调控基因。
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