Rutberg L
J Virol. 1969 Jan;3(1):38-44. doi: 10.1128/JVI.3.1.38-44.1969.
Bacteriophage phi105 is a temperate bacteriophage for Bacillus subtilis 168. Temperature-sensitive and plaque mutants of phi105 were isolated. The results of two- and three-factor crosses with these mutants suggest the vegetative map of phi105 to be circular. The location of prophage phi105 between bacterial markers phe-1 and ilvA1 was shown by means of PBS1 transduction. Five markers in the prophage were linearly ordered with respect to the bacterial markers. Linkage between bacterial and prophage markers was demonstrated in transformation experiments with deoxyribonucleic acid extracted from lysogenic bacteria. The data demonstrate that prophage phi105 is linearly inserted into the bacterial chromosome.
噬菌体phi105是枯草芽孢杆菌168的一种温和噬菌体。分离出了phi105的温度敏感型和噬菌斑突变体。用这些突变体进行的双因子和三因子杂交结果表明，phi105的营养图谱是环状的。通过PBS1转导显示了原噬菌体phi105在细菌标记phe-1和ilvA1之间的位置。原噬菌体中的五个标记相对于细菌标记呈线性排列。在用从溶源菌中提取的脱氧核糖核酸进行的转化实验中，证明了细菌和原噬菌体标记之间的连锁关系。数据表明，原噬菌体phi105是线性插入细菌染色体的。
