Institute for Vascular Signalling, Centre of Molecular Medicine, Goethe University, Frankfurt am Main D-60596, Germany; German Center of Cardiovascular Research (DZHK), Partner site RheinMain, Frankfurt am Main, Germany.
Institute for Vascular Signalling, Centre of Molecular Medicine, Goethe University, Frankfurt am Main D-60596, Germany.
Metabolism. 2019 Jan;90:16-19. doi: 10.1016/j.metabol.2018.10.008. Epub 2018 Oct 24.
Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disease associated with insulin resistance and increased risk of cardiovascular diseases. However, a biomarker for potential cardiovascular disease in PCOS patients is not available.
Twenty-two patients with PCOS and 22 healthy controls were included in the present study and amino acid profiling was performed on fasting plasma samples. Circulating microparticles were characterized by FACS analysis and complemented with enzyme activity assays.
The ratio of ornithine to arginine was significantly increased in plasma form PCOS patients and was associated with a significant increase in plasma arginase levels and activity. Platelet-derived microparticles were identified to be the main sources of the increased plasma arginase activity.
Increased levels of arginase-bearing platelet-derived microparticles contribute to the alteration of the arginine metabolism in patients with polycystic ovary syndrome. Moreover, ornithine and arginine levels represent early biomarkers of potential cardiovascular disease in PCOS patients.
多囊卵巢综合征（PCOS）是一种与胰岛素抵抗和心血管疾病风险增加相关的内分泌和代谢疾病。然而，目前尚无用于预测 PCOS 患者心血管疾病的生物标志物。
本研究纳入了 22 名 PCOS 患者和 22 名健康对照者，并对其空腹血浆样本进行了氨基酸谱分析。通过流式细胞术分析对循环微颗粒进行了特征描述，并辅以酶活性测定。
PCOS 患者的血浆中鸟氨酸/精氨酸比值显著升高，且与血浆精氨酸酶水平和活性的显著升高相关。血小板衍生的微颗粒被确定为增加的血浆精氨酸酶活性的主要来源。
携带精氨酸酶的血小板衍生微颗粒水平升高导致多囊卵巢综合征患者的精氨酸代谢发生改变。此外，鸟氨酸和精氨酸水平可作为 PCOS 患者潜在心血管疾病的早期生物标志物。
