State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Shanghai Pharmaceutical School, Shanghai, 200135, China.
Environ Sci Pollut Res Int. 2018 Dec;25(36):36278-36286. doi: 10.1007/s11356-018-3342-y. Epub 2018 Oct 27.
Continuous exposure of chemicals could cause various environmental impacts. Decabromodiphenyl ether (BDE209) and lead (Pb) can co-exist and are discharged simultaneously at e-waste recycling sites (EWRSs). Extensive concerns have been attracted by their toxic effects on soil microorganisms. Thus, by using high-throughput sequencing, this study explored bacterial community responses in a soil system after repeated Pb exposure in the presence of BDE209 in the laboratory during 90-day indoor incubation period. Gene sequencing of 16S rDNA performed on an Illumina MiSeq platform proved that one-off Pb exposure caused higher microbial abundance and community diversity. Additionally, both repetitive Pb treatment and exogenous BDE209 input could change bacterial community composition. Twenty-three different bacterial phyla were detected in the soil samples, while more than 90% of the sequences in each treatment belonged to a narrow variety. The sequence analyses elucidated that Proteobacteria, Acidobacteria, and Bacteroidetes were the top three dominant phyla. Our observations could provide a few insights into the ecological risks of Pb and BDE209 co-existed contamination in soils at EWRSs.
持续暴露于化学物质可能会对环境造成各种影响。十溴二苯醚（BDE209）和铅（Pb）可以共存并在电子废物回收站点（EWRS）中同时排放。它们对土壤微生物的毒性作用引起了广泛关注。因此，本研究通过高通量测序，在实验室 90 天室内培养期间，研究了重复 Pb 暴露存在 BDE209 时土壤系统中细菌群落的反应。Illumina MiSeq 平台上的 16S rDNA 基因测序表明，一次性 Pb 暴露会导致更高的微生物丰度和群落多样性。此外，重复 Pb 处理和外源 BDE209 的输入都可以改变细菌群落组成。在土壤样品中检测到 23 种不同的细菌门，而每个处理中的序列超过 90%属于少数几种。序列分析表明，变形菌门、酸杆菌门和拟杆菌门是前三大优势门。我们的观察结果可以为 EWRS 土壤中 Pb 和 BDE209 共存污染的生态风险提供一些见解。
