Wasilenko W J, Shawver L K, Weber M J
J Cell Physiol. 1987 Jun;131(3):450-7. doi: 10.1002/jcp.1041310318.
The effects of src oncogene expression on epidermal growth factor (EGF) receptors have been investigated in mouse 3T3 and rat-1 fibroblasts. Transformation of both cell types with src resulted in marked reductions in cellular EGF receptor levels, as assayed by either 125I-EGF binding or immunoprecipitation of receptor protein from radiolabeled cell lysates. In contrast to cells transformed by other types of retroviral oncogenes, the loss of EGF receptors in the src-transformed cells did not appear to be due to secreted transforming growth factor-alpha (TGF-alpha), since such factors were undetectable in culture fluids from the src-transformed cells. By several criteria of transformation, an EGF-receptorless cell line infected with src was shown to be transformed, suggesting that EGF receptors themselves are not obligatory to the src transformation process. We suggest that pp60src down-modulates EGF receptors by an intracellular mechanism and that the loss of the receptors is symptomatic of more general effects of pp60src on the machinery of growth regulation.
src癌基因表达对表皮生长因子（EGF）受体的影响已在小鼠3T3和大鼠-1成纤维细胞中进行了研究。用src对这两种细胞类型进行转化，导致细胞EGF受体水平显著降低，这是通过125I-EGF结合或从放射性标记的细胞裂解物中免疫沉淀受体蛋白来测定的。与由其他类型逆转录病毒癌基因转化的细胞不同，src转化细胞中EGF受体的丧失似乎不是由于分泌的转化生长因子-α（TGF-α），因为在src转化细胞的培养液中检测不到此类因子。根据几种转化标准，感染src的无EGF受体细胞系被证明发生了转化，这表明EGF受体本身对于src转化过程并非必不可少。我们认为pp60src通过细胞内机制下调EGF受体，并且受体的丧失是pp60src对生长调节机制更广泛影响的症状表现。
