Shin Sanghyun, Son Jae-Han, Park Jong-Chul, Kim Kyeong-Hoon, Yoon Young-Mi, Cheong Young-Keun, Kim Kyong-Ho, Hyun Jong-Nae, Park Chul Soo, Dill-Macky Ruth, Kang Chon-Sik
National Institute of Crop Science, RDA, Wanju 55365, Korea.
Department of Crop Science and Biotechnology, Jeonbuk National University, Jeonju 54896, Korea.
Plant Pathol J. 2018 Oct;34(5):347-355. doi: 10.5423/PPJ.OA.01.2018.0013. Epub 2018 Oct 1.
Fusarium head blight (FHB) caused by species is a major disease of wheat and barley around the world. FHB causes yield reductions and contamination of grains with trichothecene mycotoxins including; nivalenol (NIV), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), and 15-acetylde-oxynivalenol (15-ADON). The objectives of this study were to identify strains of isolated in Korea from 2012-harvested wheat grain and to test the pathogenicity of these NIV- and DON-producing isolates. Three hundred and four samples of wheat grain, harvested in 2012 in Chungnam, Chungbuk, Gyeongnam, Jeonbuk, Jeonnam, and Gangwon provinces were collected. We recovered 44 isolates from the 304 samples, based on the PCR amplification of internal transcribed spacer (ITS) rRNA region and sequencing. Our findings indicate that was the predominant (95% of all isolates) species in Korea. We recovered both and from samples collected in Chungnam province. Of the 44 isolates recovered, 36 isolates had a NIV genotype while 8 isolates belonged to the DON genotype (3-ADON and 15-ADON). In order to characterize the pathogenicity of the strains collected, disease severity was assessed visually on various greenhouse-grown wheat cultivars inoculated using both NIV- and DON-producing isolates. Our results suggest that Korean isolates from wheat belong to producing NIV, and both and are not significantly different on virulence in wheat cultivars.
由镰刀菌属物种引起的小麦赤霉病（FHB）是全球小麦和大麦的主要病害。FHB会导致产量降低，以及谷物被单端孢霉烯族霉菌毒素污染，包括：雪腐镰刀菌烯醇（NIV）、脱氧雪腐镰刀菌烯醇（DON）、3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）和15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON）。本研究的目的是鉴定2012年从韩国收获的小麦籽粒中分离出的镰刀菌菌株，并测试这些产生NIV和DON的分离株的致病性。采集了2012年在忠南、忠北、庆南、全北、全南和江原道收获的304份小麦籽粒样本。基于内部转录间隔区（ITS）rRNA区域的PCR扩增和测序，我们从304个样本中分离出44株菌株。我们的研究结果表明，在韩国，禾谷镰刀菌是主要的（占所有分离株的95%）物种。我们从忠南省采集的样本中分离出了禾谷镰刀菌和黄色镰刀菌。在分离出的44株菌株中，36株具有NIV基因型，而8株属于DON基因型（3-ADON和15-ADON）。为了表征所采集菌株的致病性，在使用产生NIV和DON的分离株接种的各种温室种植的小麦品种上，通过肉眼评估病害严重程度。我们的结果表明，从韩国小麦中分离出的禾谷镰刀菌菌株属于产生NIV的类型，并且禾谷镰刀菌和黄色镰刀菌在小麦品种上的毒力没有显著差异。
