Ahmad M H, Shakeel M T, Al-Shahwan I M, Al-Saleh M A, Amer M A
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Department of Plant Pathology, Bahauddin Zakariya University, Multan, Pakistan.
Plant Pathol J. 2018 Oct;34(5):426-434. doi: 10.5423/PPJ.OA.04.2018.0059. Epub 2018 Oct 1.
During the spring season of 2014, a total of 148 melon and watermelon leaf samples were collected from symptomatic and asymptomatic plants in the western and southwestern regions of Saudi Arabia and were tested for the presence of (WmCSV) and other suspected cucurbit viruses by double antibody sandwich enzyme-linked immunosorbent assays. Ninety-eight samples were found to be positive for the presence of WmCSV, nine samples were positive for the presence of (CYSDV), and 22 showed a mixed infection with both WmCSV and CYSDV. No other cucurbit viruses were detected in any of the samples. Host range experiments revealed that eight out of fourteen tested plant species were susceptible to WmCSV. PCR products of approximately 1.2 kb were obtained after amplification using primers specifically targeting the coat protein region of WmCSV. Positive PCR results were confirmed by dot blot hybridization. Coat protein gene sequences from eleven WmCSV isolates indicated that the highest identity was between the 104WMA-SA isolate from the Wadi Baish location and a previously reported isolate from the AL-Lith location in Saudi Arabia. The lowest identity was observed between the 42WMA-SA isolate and an isolate from Palestine.
2014年春季，从沙特阿拉伯西部和西南部有症状及无症状的甜瓜和西瓜植株上共采集了148份叶片样本，通过双抗体夹心酶联免疫吸附测定法检测西瓜褪绿斑驳病毒（WmCSV）及其他疑似葫芦科病毒的存在情况。发现98份样本WmCSV呈阳性，9份样本黄瓜黄化斑驳病毒（CYSDV）呈阳性，22份样本同时感染了WmCSV和CYSDV。在任何样本中均未检测到其他葫芦科病毒。寄主范围试验表明，受试的14种植物中有8种对WmCSV敏感。使用特异性靶向WmCSV外壳蛋白区域的引物进行扩增后，获得了约1.2 kb的PCR产物。PCR阳性结果通过斑点杂交得以证实。来自11个WmCSV分离株的外壳蛋白基因序列表明，来自瓦迪拜什地区的104WMA-SA分离株与沙特阿拉伯以前报道的来自利思地区的分离株之间的同源性最高。42WMA-SA分离株与来自巴勒斯坦的一个分离株之间的同源性最低。
