Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod 123, Sultanate of Oman.
Viruses. 2012 Jul;4(7):1169-81. doi: 10.3390/v4071169. Epub 2012 Jul 24.
Watermelon chlorotic stunt virus (WmCSV) is a bipartite begomovirus (genus Begomovirus, family Geminiviridae) that causes economic losses to cucurbits, particularly watermelon, across the Middle East and North Africa. Recently squash (Cucurbita moschata) grown in an experimental field in Oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. Sequence analysis of the virus isolated from squash showed 97.6-99.9% nucleotide sequence identity to previously described WmCSV isolates for the DNA A component and 93-98% identity for the DNA B component. Agrobacterium-mediated inoculation to Nicotiana benthamiana resulted in the development of symptoms fifteen days post inoculation. This is the first bipartite begomovirus identified in Oman. Overall the Oman isolate showed the highest levels of sequence identity to a WmCSV isolate originating from Iran, which was confirmed by phylogenetic analysis. This suggests that WmCSV present in Oman has been introduced from Iran. The significance of this finding is discussed.
西瓜曲叶黄化病毒（WmCSV）是一种二分体伴随病毒（属伴随病毒，家族双生病毒科），在中东和北非地区导致葫芦科作物，特别是西瓜遭受经济损失。最近，在阿曼的一个实验田中发现种植的南瓜（Cucurbita moschata）出现叶片卷曲、黄化和矮化等症状，这是伴随病毒感染的典型特征。从南瓜中分离出的病毒序列分析显示，其 DNA A 组分与先前描述的 WmCSV 分离株的核苷酸序列同源性为 97.6-99.9%，而 DNA B 组分的同源性为 93-98%。农杆菌介导的接种到 Nicotiana benthamiana 上导致接种后十五天出现症状。这是在阿曼首次发现的二分体伴随病毒。总体而言，阿曼分离株与源自伊朗的 WmCSV 分离株的序列同一性最高，这通过系统发育分析得到了证实。这表明，在阿曼存在的 WmCSV 是从伊朗引入的。讨论了这一发现的意义。
