Brandt Nina, Kotowska Dorota, Kristensen Caroline M, Olesen Jesper, Lützhøft Ditte O, Halling Jens F, Hansen Martin, Al-Soud Waleed A, Hansen Lars, Kiilerich Pia, Pilegaard Henriette
Department of Biology, University of Copenhagen, Copenhagen Ø, Denmark.
Department of Veteranary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Physiol Rep. 2018 Oct;6(20):e13881. doi: 10.14814/phy2.13881.
The aim of this study was to examine the effect of exercise training and dietary supplementation of resveratrol on the composition of gut microbiota and to test the hypothesis that exercise training and resveratrol can prevent high-fat diet (HFD)-induced changes in the gut microbiota. Mice fed a HFD supplemented with resveratrol (4 g/kg food) were protected against diet-induced obesity, while exercise trained HFD-fed animals (running on average 50 km/week) were not. Dietary resveratrol supplementation induced changes predominantly in the low-abundant bacteria, while exercise training induced changes in the high-abundant bacteria in the gut as analyzed by ADONIS test with Weighted UniFrac distances. Interestingly, the two interventions affected the gut microbiome independently of the inflammatory state of the HFD-fed animals as assessed by the systemic serum amyloid A levels. These results suggest that both resveratrol supplementation and regular physical activity modulate the composition of murine microbiota independently of the systemic inflammatory state. Moreover, the effects of exercise training on the microbiota seem to occur without changes in adiposity, while resveratrol-mediated alterations may relate to adipose tissue mass.
本研究的目的是探讨运动训练和白藜芦醇膳食补充剂对肠道微生物群组成的影响，并验证运动训练和白藜芦醇可预防高脂饮食（HFD）诱导的肠道微生物群变化这一假设。喂食添加白藜芦醇（4克/千克食物）的高脂饮食的小鼠可预防饮食诱导的肥胖，而接受运动训练的高脂饮食喂养动物（平均每周跑步50公里）则不然。通过基于加权UniFrac距离的ADONIS检验分析，膳食补充白藜芦醇主要引起低丰度细菌的变化，而运动训练则引起肠道中高丰度细菌的变化。有趣的是，通过全身血清淀粉样蛋白A水平评估，这两种干预措施对高脂饮食喂养动物的肠道微生物群的影响与炎症状态无关。这些结果表明，补充白藜芦醇和定期体育活动均可独立于全身炎症状态调节小鼠微生物群的组成。此外，运动训练对微生物群的影响似乎在肥胖无变化的情况下发生，而白藜芦醇介导的改变可能与脂肪组织量有关。
