Nakamura Yoshiaki, Nakane Yoshifumi, Tsudzuki Masaoki
Laboratory of Animal Breeding and Genetics, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan.
Japanese Avian Bioresource Project Research Center, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan.
Anim Sci J. 2019 Jan;90(1):35-48. doi: 10.1111/asj.13119. Epub 2018 Oct 28.
Chickens and Japanese quail (Coturnix japonica) have traditionally been the primary avian models in developmental biology research. Recently, the blue-breasted quail (Coturnix chinesis), the smallest species in the order Galliformes, has been proposed as an excellent candidate model in avian developmental studies owing to its precocious and prolific properties. However, data on the embryonic development of blue-breasted quail are scarce. Here, we developed a normal developmental series for the blue-breasted quail based on developmental features. The blue-breasted quail embryos take 17 days to reach the hatching period at 37.7°C. We documented specific periods of incubation in which significant development occurred, and created a 39-stage developmental series. The developmental series for the blue-breasted quail was almost identical to that for chickens and Japanese quail in the earlier stages of development (stages 1-16). Our staging series is especially useful at later stages of development (stages 34-39) of blue-breasted quail embryos as a major criterion of staging in this phase of development was the weight of embryos and the length of third toes.
鸡和日本鹌鹑（Coturnix japonica）一直是发育生物学研究中的主要禽类模型。最近，蓝胸鹑（Coturnix chinesis）作为鸡形目中最小的物种，因其早熟和繁殖力强的特性，被提议作为禽类发育研究中的优秀候选模型。然而，关于蓝胸鹑胚胎发育的数据却很稀少。在此，我们基于发育特征为蓝胸鹑建立了一个正常发育序列。蓝胸鹑胚胎在37.7°C下需要17天才能达到孵化期。我们记录了发生显著发育的特定孵化期，并创建了一个39阶段的发育序列。蓝胸鹑的发育序列在发育早期阶段（1 - 16阶段）与鸡和日本鹌鹑的几乎相同。我们的分期序列在蓝胸鹑胚胎发育的后期阶段（34 - 39阶段）特别有用，因为在这个发育阶段分期的一个主要标准是胚胎的重量和第三趾的长度。
