University of Louisville, Department of Pathology, University of Louisville, Louisville, KY 40202, USA.
Institute of Biochemistry, School of Nutritional Sciences, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Int J Mol Sci. 2018 Oct 28;19(11):3371. doi: 10.3390/ijms19113371.
is recognized for its prominent nutritional and medicinal value. In our study, we tested the effect of glucans on lipopolysaccharide (LPS)-induced production of TNF-α. We demonstrated that glucan extracts are more effective than mill mushroom preparations. Additionally, the effectiveness of stalk-derived glucans were slightly more pronounced than of caps. Cap and stalk glucans from mill or isolated glucan competed dose-dependently with anti-Dectin-and anti-CR-3 antibodies, indicating that they contain β-glucans recognized by these receptors. Using the dextran sulfate sodium (DSS)-inflammatory bowel disease mice model, intestinal inflammatory response to the mill preparations was measured and compared to extracted glucan fractions from caps and stalks. We found that mill and glucan extracts were very effective in downregulating IFN-γ and MIP-2 levels and that stalk-derived preparations were more effective than from caps. The tested glucans were equally effective in regulating the number of CD14/CD16 monocytes and upregulating the levels of fecal-released IgA to almost normal levels. In conclusion, the most effective glucans in ameliorating some IBD-inflammatory associated symptoms induced by DSS treatment in mice were glucan extracts prepared from the stalk of . These spatial distinctions may be helpful in selecting more effective specific anti-inflammatory mushrooms-derived glucans.
灵芝因其显著的营养和药用价值而受到认可。在我们的研究中，我们测试了葡聚糖对脂多糖（LPS）诱导的 TNF-α产生的影响。我们证明，葡聚糖提取物比磨菇制剂更有效。此外，菌柄来源的葡聚糖的有效性略高于菌盖。来自磨菇或分离的葡聚糖的菌盖和菌柄葡聚糖与抗 Dectin 和抗 CR-3 抗体竞争性结合，表明它们含有这些受体识别的β-葡聚糖。使用葡聚糖硫酸钠（DSS）-炎症性肠病小鼠模型，测量了对磨菇制剂的肠道炎症反应，并与菌盖和菌柄的提取葡聚糖级分进行了比较。我们发现磨菇和葡聚糖提取物在下调 IFN-γ和 MIP-2 水平方面非常有效，并且菌柄来源的制剂比菌盖来源的更有效。测试的葡聚糖在调节 CD14/CD16 单核细胞数量和上调粪便中释放的 IgA 水平方面同样有效，几乎达到正常水平。总之，在减轻 DSS 处理诱导的一些 IBD-炎症相关症状方面，最有效的葡聚糖是从灵芝菌柄制备的葡聚糖提取物。这些空间差异可能有助于选择更有效的特定抗炎蘑菇来源的葡聚糖。
