Salzburg University of Applied Sciences, Biomedical Sciences, Urstein Süd 1, Puch/Salzburg 5412, Austria.
Biomedical Sciences, Salzburg University of Applied Sciences, Salzburg, Austria.
Clin Chem Lab Med. 2019 May 27;57(6):891-900. doi: 10.1515/cclm-2018-0715.
Background Reference intervals are a prerequisite for the interpretation of laboratory data related to diagnostic issues and treatment strategies. In adolescents, biomarker concentrations change with age, necessitating a continuous age-related definition of the reference intervals. The purpose of this pilot study was to evaluate the reference intervals for a healthy population of adolescents in Salzburg and compare these, when possible, with age- and gender-matched published data. Methods Anthropometrical parameters and blood samples were collected from adolescents (male and female; 14-17 years) in a school setting. Haematological samples were measured using Sysmex XS-1000i, lipid and carbohydrate metabolism markers as well as enzymes and hormones were determined by Cobas c311, Vitros ECiQ® or ELISA. The reference intervals were calculated according to the CLSI guidelines C28-A3c. Results Samples of 102 participants were included. Compared to age- and gender-matched reference intervals, the BMI levels were in the lower normal rage. Most haematological parameters and biomedical makers reveal similar ranges to values published in other studies. Conclusions This data analysis allowed for a partial comparison of reference values with published data and enabled a new determination of paediatric reference intervals for an Austrian cohort.
背景参考区间是解释与诊断问题和治疗策略相关的实验室数据的前提。在青少年中，生物标志物浓度随年龄而变化，因此需要不断定义与年龄相关的参考区间。本初步研究旨在评估萨尔茨堡健康青少年人群的参考区间，并在可能的情况下将其与年龄和性别匹配的已发表数据进行比较。方法在学校环境中收集青少年（男性和女性；14-17 岁）的人体测量参数和血液样本。使用 Sysmex XS-1000i 测量血液样本，使用 Cobas c311、Vitros ECiQ®或 ELISA 测定脂质和碳水化合物代谢标志物以及酶和激素。参考区间根据 CLSI 指南 C28-A3c 计算。结果纳入了 102 名参与者的样本。与年龄和性别匹配的参考区间相比，BMI 水平处于正常低值。大多数血液学参数和生物医学标志物的范围与其他研究中公布的值相似。结论本数据分析允许与已发表数据的部分参考值进行比较，并为奥地利队列确定了新的儿科参考区间。
