Department of Clinical Laboratory, Peking University First Hospital, Beijing, 100034, China.
Department of Central Laboratory, Peking University Shougang Hospital, Beijing, 100144, China.
Biochem Biophys Res Commun. 2018 Nov 30;506(3):674-679. doi: 10.1016/j.bbrc.2018.10.152. Epub 2018 Oct 28.
Urothelial Carcinoma Antigen 1 (UCA1) is a cell and tissue specific long non-coding RNA (lncRNA) associated with the tumorigenesis and invasion of bladder cancer. However, the mechanism driving the over-transcription of UCA1 in bladder cancer cells remains unclear. It has been reported that C/EBPβ has a significant role of regulation in tumorigenesis. Here we report that the expression of UCA1 was dramatically inhibited in 5637 cells with C/EBPβ down-regulation. Additionally, the function tests indicated that C/EBPβ could promote 5637 cells growth and colony formation by inducing the expression level of UCA1. These data suggest that C/EBPβ was involved in transcriptional regulation of UCA1 and contributed substantially to its high expression and proliferation promoting in bladder cancer cells.
尿路上皮癌相关抗原 1（UCA1）是一种与膀胱癌的发生和侵袭相关的细胞和组织特异性长链非编码 RNA（lncRNA）。然而，驱动膀胱癌细胞中 UCA1 过度转录的机制尚不清楚。据报道，C/EBPβ 在肿瘤发生中具有重要的调节作用。在这里，我们报告说，在下调 C/EBPβ 的 5637 细胞中，UCA1 的表达明显受到抑制。此外，功能测试表明，C/EBPβ 可以通过诱导 UCA1 的表达水平来促进 5637 细胞的生长和集落形成。这些数据表明，C/EBPβ 参与了 UCA1 的转录调控，并在膀胱癌细胞中高表达和促进增殖方面发挥了重要作用。
