丙型肝炎病毒检测方法的研究。
Study of Hepatitis C Virus Detection Assays.
作者信息
Khudur Al-Nassary Mithaq Sabeeh, Mahdi Batool Mutar
机构信息
Al-Kindy Teaching Hospital, Laboratory Department, Iraq.
Al-Kindy College of Medicine, Head of HLA Research Unit, University of Baghdad, Iraq.
出版信息
Ann Med Surg (Lond). 2018 Oct 6;36:47-50. doi: 10.1016/j.amsu.2018.10.002. eCollection 2018 Dec.
BACKGROUND
Hepatitis C virus is a small, enveloped, positive-sense single-stranded RNA virus that causes and liver cancer like hepatocellular carcinoma and lymphomas.: to assess different methods in diagnosis HCV infection.
PATIENTS AND METHODS
A retrospective study of 426 patients was admitted to Al-Kindy Teaching Hospital, Baghdad-Iraq for surgical operations or renal dialysis from January-2015 to December-2016. Their serum tested for HCV Abs by rapid immunochromatography, Enzyme Linked ImunoSorbent Assay (ELISA), and RIBA test.
RESULTS
The study sample was 426 patients, their age was ranged from 15 to 65 years. Males were represented 58% and the rest were females. The serum of all samples has tested by rapid Immunochromatography test. Fifty percent of them showed positive results by this test and the rest were negative. Those fifty serum samples who were positive by Immunochromatography test were reexamined by ELISA test and showed 39out of 50 (78%) were true positive by ELISA test and the rest were negative (P = 0.0001).The positive samples by ELISA have tested by RIBA test that showed 200(80%)were true positive in males and 130(74%)were true positive in females and the rest were false positive (P = 0.0001).
CONCLUSIONS
Early screening of the high risk group of population by highly sensitive test is important to treat infected patients and prevent dissemination among population.
背景
丙型肝炎病毒是一种小型包膜正链单链RNA病毒，可引发肝细胞癌和淋巴瘤等肝癌。目的：评估诊断丙型肝炎病毒感染的不同方法。
患者和方法
对2015年1月至2016年12月因外科手术或肾透析入住伊拉克巴格达金迪教学医院的426例患者进行回顾性研究。通过快速免疫层析法、酶联免疫吸附测定（ELISA）和重组免疫印迹试验（RIBA）检测他们血清中的丙肝抗体。
结果
研究样本为426例患者，年龄在15至65岁之间。男性占58%，其余为女性。所有样本的血清均通过快速免疫层析试验进行检测。其中50%通过该试验显示阳性结果，其余为阴性。对免疫层析试验呈阳性的50份血清样本进行ELISA试验复检，结果显示50份中有39份（78%）通过ELISA试验为真阳性，其余为阴性（P = 0.0001）。ELISA试验呈阳性的样本通过RIBA试验检测，结果显示男性中有200份（80%）为真阳性，女性中有130份（74%）为真阳性，其余为假阳性（P = 0.0001）。
结论
通过高灵敏度检测对高危人群进行早期筛查对于治疗感染患者和防止病毒在人群中传播非常重要。
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