Zhang Yuchan, Wang Xiaowen, Wang Xinyu, Wang Yukang, Liu Jun, Wang Saisai, Li Weiran, Jin Yijun, Akhter Delara, Chen Jiarong, Hu Jianping, Pan Ronghui
College of Agriculture and Biotechnology & ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, China.
Zhejiang Lab, Hangzhou, China.
Front Plant Sci. 2023 Jun 9;14:1180647. doi: 10.3389/fpls.2023.1180647. eCollection 2023.
Peroxisomes are ubiquitous eukaryotic organelles housing not only many important oxidative metabolic reactions, but also some reductive reactions that are less known. Members of the short-chain dehydrogenase/reductase (SDR) superfamily, which are NAD(P)(H)-dependent oxidoreductases, play important roles in plant peroxisomes, including the conversion of indole-3-butyric acid (IBA) to indole-3-acetic acid (IAA), auxiliary β-oxidation of fatty acids, and benzaldehyde production. To further explore the function of this family of proteins in the plant peroxisome, we performed an search for peroxisomal SDR proteins from based on the presence of peroxisome targeting signal peptides. A total of 11 proteins were discovered, among which four were experimentally confirmed to be peroxisomal in this study. Phylogenetic analyses showed the presence of peroxisomal SDR proteins in diverse plant species, indicating the functional conservation of this protein family in peroxisomal metabolism. Knowledge about the known peroxisomal SDRs from other species also allowed us to predict the function of plant SDR proteins within the same subgroup. Furthermore, gene expression profiling revealed strong expression of most genes in floral tissues and during seed germination, suggesting their involvement in reproduction and seed development. Finally, we explored the function of SDRj, a member of a novel subgroup of peroxisomal SDR proteins, by generating and analyzing CRISPR/Cas mutant lines. This work provides a foundation for future research on the biological activities of peroxisomal SDRs to fully understand the redox control of peroxisome functions.
过氧化物酶体是普遍存在的真核细胞器，不仅包含许多重要的氧化代谢反应，还包括一些鲜为人知的还原反应。短链脱氢酶/还原酶（SDR）超家族的成员是依赖NAD(P)(H)的氧化还原酶，在植物过氧化物酶体中发挥重要作用，包括将吲哚-3-丁酸（IBA）转化为吲哚-3-乙酸（IAA）、脂肪酸的辅助β-氧化以及苯甲醛的生成。为了进一步探索该蛋白家族在植物过氧化物酶体中的功能，我们基于过氧化物酶体靶向信号肽的存在，从[具体物种]中搜索过氧化物酶体SDR蛋白。共发现11种蛋白，其中4种在本研究中经实验证实定位于过氧化物酶体。系统发育分析表明不同植物物种中均存在过氧化物酶体SDR蛋白，这表明该蛋白家族在过氧化物酶体代谢中功能保守。对其他物种已知的过氧化物酶体SDR的了解也使我们能够预测同一亚组中植物SDR蛋白的功能。此外，[具体物种]基因表达谱显示大多数[具体物种]基因在花组织和种子萌发过程中强烈表达，表明它们参与繁殖和种子发育。最后，我们通过构建和分析CRISPR/Cas突变体株系，探索了过氧化物酶体SDR蛋白新亚组成员SDRj的功能。这项工作为未来研究过氧化物酶体SDR的生物学活性以全面了解过氧化物酶体功能的氧化还原调控奠定了基础。
