Li Fu-Min, Liu Yuan, Liao Jin-Feng, Duan Xi-Ling
Department of Dermatology, Sichuan Provincial People's Hospital Sichuan Academy of Medical Sciences, Chengdu 610072, China.
Graduate School, Southwest Medical University, Luzhou 646000, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 Sep;49(5):712-715.
To study the protective effects of astaxanthin liposome (Asx-lipo) on photodamage by UVB in mice skin.
40 C57BL/6J mice were randomly divided into four groups: The blank group (no irradiation, no drug use), model group (UVB light injury group, no drug use), control group (irradiation + astaxanthin), experimental group (irradiation + astaxanthin liposome), each group with 10 mice. Each group was given the corresponding light (the radiation intensity was 2 mW·cm, the time of irradiation was 60 s, 1 times a day for the first 5 days, and 1 times every other day for the next 9 days, 10 times in a total of 2 weeks.) and drug intervention (topically treated with 4 mL 0.2‰ astaxanthin or 4 mL 0.2‰ Asx-lipo 10 min before the irradiation) for two weeks. After that, samples were examined by the following indicators: the histological changes of skin, Ki-67, 8-hydroxy-2'-deoxyguanosine(8-OHdG), superoxide dismutase(SOD) activities and serum matrix metalloproteinase-13 (MMP-13).
HE staining the model group and the control group showed that the dermis became thin, the dermal collagen fibers were long and thin, and the arrangement was loose and disordered. Compared with the blank group, the expression of Ki-67, MMP-13 and 8-OHdG increased and SOD activity decreased, and the differences were statistically significant (<0.05). Compared with the model group, the pathological changes of skin tissues in the experimental group were significantly improved, with decreased expressions of Ki-67, MMP-13 and 8-OHdG and increased SOD activity, and the differences were statistically significant (<0.05).
The photodamage of mice skin can be improved by topical Asx-lipo. The mechanism may be related to the strong antioxidation of Asx-lipo.
研究虾青素脂质体（Asx-lipo）对小鼠皮肤紫外线B（UVB）光损伤的保护作用。
将40只C57BL/6J小鼠随机分为四组：空白组（不照射，不使用药物）、模型组（UVB光损伤组，不使用药物）、对照组（照射+虾青素）、实验组（照射+虾青素脂质体），每组10只。每组给予相应光照（辐射强度为2 mW·cm，照射时间为60 s，前5天每天1次，后9天隔天1次，共2周照射10次）和药物干预（照射前10 min局部涂抹4 mL 0.2‰虾青素或4 mL 0.2‰ Asx-lipo），持续两周。之后，通过以下指标进行检测：皮肤组织学变化、Ki-67、8-羟基-2'-脱氧鸟苷（8-OHdG）、超氧化物歧化酶（SOD）活性以及血清基质金属蛋白酶-13（MMP-13）。
模型组和对照组的苏木精-伊红（HE）染色显示真皮变薄，真皮胶原纤维细长，排列疏松紊乱。与空白组相比，模型组和对照组的Ki-67、MMP-13和8-OHdG表达增加，SOD活性降低，差异具有统计学意义（<0.05）。与模型组相比，实验组皮肤组织的病理变化明显改善，Ki-67、MMP-13和8-OHdG表达降低，SOD活性增加，差异具有统计学意义（<0.05）。
局部应用Asx-lipo可改善小鼠皮肤的光损伤。其机制可能与Asx-lipo的强抗氧化作用有关。
