Cui Lili, Yan Meixia, Piao Xiangmin, Pang Shifeng, Wang Yingping
Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China.
Se Pu. 2018 Nov 1;36(11):1173-1180. doi: 10.3724/SP.J.1123.2018.06015.
In this study, the extraction and purification improvements of the QuEChERS method were coupled with gas chromatography-mass spectrometry (GC-MS) to determine 20 pesticides in . Dry ginseng powder was mixed with water, and the solution was then extracted with acetonitrile and purified with -propylethylenediamine (PSA) and MgSO to remove co-extractives that might interfere with the results. The target compounds were detected after electron-impact ionization in selected ion monitoring (SIM) mode. Under optimum conditions, the method gave a good linear relationship for the determination of the pesticides within a certain concentration range, with correlation coefficients greater than 0.990. Moreover, the limits of detection (=3) were 0.002-0.007 mg/kg, the limits of quantification (=10) were 0.002-0.024 mg/kg, and the average recoveries for pesticides in were 70.41%-114.06%, with relative standard deviations of 0.76%-15.47%. In comparison with previous methods, the new procedure has the advantages of simple sample preparation and higher sensitivity.
在本研究中，对QuEChERS方法的提取和净化进行了改进，并与气相色谱 - 质谱联用仪（GC-MS）相结合，以测定[具体物质]中的20种农药。将干燥的人参粉末与水混合，然后用乙腈萃取该溶液，并用丙基乙二胺（PSA）和硫酸镁（MgSO）进行净化，以去除可能干扰结果的共萃取物。在选择离子监测（SIM）模式下通过电子轰击电离检测目标化合物。在最佳条件下，该方法在一定浓度范围内对农药的测定具有良好的线性关系，相关系数大于0.990。此外，检测限（n = 3）为0.002 - 0.007 mg/kg，定量限（n = 10）为0.002 - 0.024 mg/kg，[具体物质]中农药的平均回收率为70.41% - 114.06%，相对标准偏差为0.76% - 15.47%。与先前的方法相比，新方法具有样品制备简单和灵敏度更高的优点。
