西咪替丁和组胺与无细胞体系及中性粒细胞中产生的超氧化物的相互作用。

Interaction of cimetidine and histamine with superoxide generated in a cell-free system and in neutrophils.

作者信息

Tarnok I, Tarnok Z

出版信息

Agents Actions. 1987 Apr;20(3-4):324-6. doi: 10.1007/BF02074703.

DOI:10.1007/BF02074703

Abstract

We investigated the influence of CIM and HIS on the SUP and CH in the SUP generating cell-free xanthine oxidase system and in human neutrophils. As measured by CCR or CH, we found that HIS inhibited SUP output in both systems. The HIS concentration required for a measurable inhibition was somewhat higher in the xanthine oxidase system than in neutrophils. CIM increased CH in neutrophils significantly. A similar effect of CIM was not observed in the xanthine oxidase system. CIM antagonized in neutrophils the effect of HIS. However, a five or ten times higher molar concentration of CIM than of HIS was necessary to produce this effect. The antagonistic effect of CIM was not found in the xanthine oxidase system within the concentrations applied. In addition to an interaction of HIS with neutrophils on the cellular level, we suggest a superoxidase scavenging effect caused by HIS. At the present no suggestions can be made regarding the observed SUP output stimulating activity of CIM.

摘要

我们研究了氯碘羟喹（CIM）和组胺（HIS）对无细胞黄嘌呤氧化酶系统和人中性粒细胞中超氧阴离子（SUP）生成及趋化作用（CH）的影响。通过趋化反应（CCR）或趋化作用（CH）测定，我们发现HIS在两个系统中均抑制SUP输出。在黄嘌呤氧化酶系统中，产生可测量抑制作用所需的HIS浓度比在中性粒细胞中略高。CIM显著增加了中性粒细胞中的趋化作用（CH）。在黄嘌呤氧化酶系统中未观察到CIM的类似作用。CIM在中性粒细胞中拮抗HIS的作用。然而，产生这种作用所需的CIM摩尔浓度比HIS高五倍或十倍。在所应用的浓度范围内，在黄嘌呤氧化酶系统中未发现CIM的拮抗作用。除了HIS在细胞水平上与中性粒细胞的相互作用外，我们认为HIS具有超氧化物清除作用。目前，关于观察到的CIM刺激SUP输出的活性尚无定论。