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卵巢异种移植后BALB/c裸鼠和C57BL/6 SCID雌性小鼠的血液学和生化指标

Hematological and biochemical profile of BALB/c nude and C57BL/6 SCID female mice after ovarian xenograft.

作者信息

Fernandes Denilsa P, Pimentel Muriel M L, Santos Fernanda A Dos, Praxedes Érika A, Brito Parmênedes D DE, Lima Mikael A, Lelis Ivana C N G, Macedo Michelly F DE, Bezerra Marcelo B

机构信息

Laboratório de Transplantes Gonadais e Produção In Vitro de Embriões/LTG-PIVE, Departamento de Ciências Animais, Universidade Federal Rural do Semi-Árido/UFERSA, Avenida Francisco Mota, 572, 59625-900 Mossoró, RN, Brazil.

出版信息

An Acad Bras Cienc. 2018 Oct-Dec;90(4):3941-3948. doi: 10.1590/0001-3765201820180586. Epub 2018 Oct 25.

Abstract

Hematological and biochemical profile studies help to evaluate functional changes of animals used in experiments. The aim of this study was to determine the hematological and biochemical profile of immunosuppressed BALB/c nude and C57BL/6 SCID mice after bovine ovarian xenotransplantation. Therefore, a total of 74 female mice were divided into four groups: non-xenotransplanted animals, xenotransplanted animals, xenotransplanted animals treated with eCG and xenotransplanted animals treated with FSH + LH. After anesthesia, blood samples were collected and hematologic and biochemical values were evaluated. The results showed no significant differences (p ≤ 0.05) for hematological parameters between the control group and the treatment groups of both strains. However, considering the biochemical profile, it was observed an increase of AST concentrations (p ≤ 0.05) in both strains and a decrease of ALT concentrations (p ≤ 0.05) only in C57BL/6 SCID strain of the groups subjected to hormonal treatment compared with those non subjected. Additionally, the values of the renal enzymes, urea and creatinine, did not differ (p ≤ 0.05) between the groups. Our findings suggest that the xenotransplantation procedure as well as the hormonal dosages had no significant effect on the well-being of the animals considering the evaluated hematological and biochemical profile.

摘要

血液学和生化指标研究有助于评估实验所用动物的功能变化。本研究的目的是确定牛卵巢异种移植后免疫抑制的BALB/c裸鼠和C57BL/6 SCID小鼠的血液学和生化指标。因此,总共74只雌性小鼠被分为四组:未进行异种移植的动物、进行异种移植的动物、用eCG处理的异种移植动物以及用FSH + LH处理的异种移植动物。麻醉后,采集血样并评估血液学和生化值。结果显示,两个品系的对照组和治疗组之间的血液学参数无显著差异(p≤0.05)。然而,考虑生化指标,与未接受激素处理的组相比,在接受激素处理的组中,两个品系的AST浓度均升高(p≤0.05),而仅在C57BL/6 SCID品系中ALT浓度降低(p≤0.05)。此外,各组之间肾酶、尿素和肌酐的值无差异(p≤0.05)。我们的研究结果表明,考虑到所评估的血液学和生化指标,异种移植程序以及激素剂量对动物的健康没有显著影响。

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