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基于载体的新冠病毒疫苗与胞嘧啶-磷酸-鸟嘌呤寡脱氧核苷酸联合给药后Balb/c小鼠免疫反应的增强

Increase in the Immune Response in Balb/c Mice after the Co-Administration of a Vector-Based COVID-19 Vaccine with Cytosine Phosphoguanine Oligodeoxynucleotide.

作者信息

Celise Divine Ainee, Kimotho James, Kimani Josephine W, Muriithi Alex Kigundu, Odari Eddy Okoth

机构信息

Department of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), P.O. Box 62000-00200, Nairobi, Kenya.

Innovation and Technology Transfer Division, Kenya Medical Research Institute (KEMRI), P.O Box 54840-00200, Nairobi, Kenya.

出版信息

Vaccines (Basel). 2022 Dec 26;11(1):53. doi: 10.3390/vaccines11010053.

DOI:10.3390/vaccines11010053
PMID:36679896
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9864427/
Abstract

The effects of cytosine phosphoguanine oligodeoxynucleotides (CPG ODNs) on immune response have been demonstrated for different vaccines; however, such information is limited for the vector-based Coronavirus disease 2019 (COVID-19). This paper aims to demonstrate the potential effect of CPG ODNs on immunological response against the vector-based COVID-19 vaccine on Balb/c mice using a JNJ-78436735 Ad26.COV2-S recombinant as a model vaccine. A total of 18 BALB/c mice clustered into six groups were used. All groups were observed for 14- and 28-days post immunization. Qualitative determination of IgG was performed using indirect Enzyme-Linked Immunosorbent Assay (ELISA) and qPCR for cytokine profiling. A significant ( ≤ 0.001) rise in antibody response was observed for groups 3 and 4, who also showed increased expression levels of Tumor Necrosis Factor (TNF) and Interferon Gamma (IFN-γ). Immunological parameters for toxicity were normal in all treatment groups. We conclude that supplementing vector-based COVID-19 vaccines with CpG ODNs has the potential to boost the body's immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

摘要

胞嘧啶磷酸鸟嘌呤寡脱氧核苷酸（CPG ODNs）对不同疫苗免疫反应的影响已得到证实；然而，关于基于载体的2019冠状病毒病（COVID-19）疫苗的此类信息有限。本文旨在以JNJ-78436735 Ad26.COV2-S重组疫苗作为模型疫苗，证明CPG ODNs对Balb/c小鼠针对基于载体的COVID-19疫苗的免疫反应的潜在影响。总共使用了18只BALB/c小鼠，分为六组。所有组在免疫后14天和28天进行观察。使用间接酶联免疫吸附测定（ELISA）进行IgG的定性测定，并使用qPCR进行细胞因子谱分析。第3组和第4组观察到抗体反应显著升高（≤0.001），这两组还显示肿瘤坏死因子（TNF）和干扰素γ（IFN-γ）的表达水平增加。所有治疗组的毒性免疫参数均正常。我们得出结论，用CpG ODNs补充基于载体的COVID-19疫苗有可能增强机体对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染的免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/680bc6c009e1/vaccines-11-00053-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/276d7c5210ea/vaccines-11-00053-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/96d3a0ad54fa/vaccines-11-00053-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/18a0eec79df4/vaccines-11-00053-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/829ec11224c0/vaccines-11-00053-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/680bc6c009e1/vaccines-11-00053-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/276d7c5210ea/vaccines-11-00053-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/96d3a0ad54fa/vaccines-11-00053-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/18a0eec79df4/vaccines-11-00053-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/829ec11224c0/vaccines-11-00053-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/660a/9864427/680bc6c009e1/vaccines-11-00053-g005.jpg

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