Gyeongnam Bio-Health Research Support Center, Gyeongnam Branch Institute, Korea Institute of Toxicology (KIT), 17 Jeigok-gil, Jinju, 52834, Republic of Korea.
Environmental Safety Assessment Center, Gyeongnam Branch Institute, Korea Institute of Toxicology (KIT), 17 Jeigok-gil, Jinju, 52834, Republic of Korea.
Mol Biol Rep. 2024 Feb 16;51(1):305. doi: 10.1007/s11033-023-09046-4.
Pectolinarigenin (PEC) is a flavone extracted from Cirsium, and because it has anti-inflammatory properties, anti-cancer research is also being conducted. The objective of this work was to find out if PEC is involved in tumor control and which pathways it regulates in vivo and in vitro.
AGS cell lines were xenografted into BALB/c nude mice to create tumors, and PEC was administered intraperitoneally to see if it was involved in tumor control. Once animal testing was completed, tumor proteins were isolated and identified using LC-MS analysis, and gene ontology of the found proteins was performed.
Body weight and hematological measurements on the xenograft mice model demonstrated that PEC was not harmful to non-cancerous cells. We found 582 proteins in tumor tissue linked to biological reactions such as carcinogenesis and cell death signaling. PEC regulated 6 out of 582 proteins in vivo and in vitro in the same way.
Our findings suggested that PEC therapy may inhibit tumor development in gastric cancer (GC), and proteomic research gives fundamental information about proteins that may have great promise as new therapeutic targets in GC.
白杨素（PEC）是从蓟中提取的一种黄酮类化合物，由于具有抗炎特性，因此也在进行抗癌研究。本工作的目的是确定 PEC 是否参与肿瘤控制以及它在体内和体外调节哪些途径。
将 AGS 细胞系异种移植到 BALB/c 裸鼠中以形成肿瘤，并通过腹腔内给予 PEC，观察其是否参与肿瘤控制。动物试验完成后，使用 LC-MS 分析分离和鉴定肿瘤蛋白，并对发现的蛋白进行基因本体分析。
异种移植小鼠模型的体重和血液学测量表明 PEC 对非癌细胞没有危害。我们在肿瘤组织中发现了 582 种与致癌和细胞死亡信号等生物反应相关的蛋白质。PEC 在体内和体外以相同的方式调节了 582 种蛋白质中的 6 种。
我们的研究结果表明，PEC 治疗可能抑制胃癌（GC）的肿瘤发展，蛋白质组学研究为可能成为 GC 新治疗靶点的蛋白质提供了基本信息。
