Stanford Cardiovascular Institute, and Department of Medicine, Division of Cardiology, Stanford University, Stanford, California 94305, USA; email:
Department of Radiology, Stanford University, Stanford, California 94305, USA.
Annu Rev Pathol. 2019 Jan 24;14:395-419. doi: 10.1146/annurev-pathmechdis-012418-013046. Epub 2018 Oct 31.
Human induced pluripotent stem cells (iPSCs) provide a renewable supply of patient-specific and tissue-specific cells for cellular and molecular studies of disease mechanisms. Combined with advances in various omics technologies, iPSC models can be used to profile the expression of genes, transcripts, proteins, and metabolites in relevant tissues. In the past 2 years, large panels of iPSC lines have been derived from hundreds of genetically heterogeneous individuals, further enabling genome-wide mapping to identify coexpression networks and elucidate gene regulatory networks. Here, we review recent developments in omics profiling of various molecular phenotypes and the emergence of human iPSCs as a systems biology model of human diseases.
人类诱导多能干细胞(iPSCs)为细胞和分子疾病机制研究提供了可再生的患者特异性和组织特异性细胞供应。结合各种组学技术的进步,iPSC 模型可用于分析相关组织中基因、转录本、蛋白质和代谢物的表达。在过去的 2 年中,已经从数百个遗传异质个体中衍生出了大量的 iPSC 系,进一步实现了全基因组图谱绘制,以鉴定共表达网络并阐明基因调控网络。在这里,我们回顾了各种分子表型的组学分析的最新进展,以及人类 iPSCs 作为人类疾病系统生物学模型的出现。