Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Expert Rev Proteomics. 2012 Aug;9(4):379-99. doi: 10.1586/epr.12.30.
The generation of human induced pluripotent stem cells (iPSCs) from differentiated cells holds important clinical implications. Human iPSCs represent the most promising resource for regenerative medicine by enabling the use of patient-specific cells of any lineage without the need for embryonic material. However, before therapeutic applications using human iPSCs are carried out, extensive analyses are needed to assess molecular differences and similarities between human iPSCs and their natural counterparts, human embryonic stem cells. The pluralism of mechanisms acting in a biological system can be better approached by studying several elements simultaneously in an unbiased manner. This review will discuss recent genome-wide analyses of iPSCs (e.g., transcripts and epigenetics) and will introduce the huge potential of mass spectrometry-based proteomics in decoding the unique mechanisms underlying the reprogramming process and the molecular nature of cellular identity.
从分化细胞中生成人类诱导多能干细胞（iPSCs）具有重要的临床意义。人类 iPSCs 通过允许使用任何谱系的患者特异性细胞，而无需胚胎材料，代表了再生医学中最有前途的资源。然而，在使用人类 iPSCs 进行治疗应用之前，需要进行广泛的分析，以评估人类 iPSCs 与其天然对应物——人类胚胎干细胞之间的分子差异和相似性。通过以无偏倚的方式同时研究多个元素，可以更好地研究生物系统中作用的机制多样性。这篇综述将讨论 iPSCs 的全基因组分析（例如转录物和表观遗传学），并介绍基于质谱的蛋白质组学在解码重编程过程背后的独特机制和细胞身份的分子性质方面的巨大潜力。
