Rufin Marie Toghueo Kouipou, Mbetyoumoun Mfouapon Heroine, Madiesse Kemgne Eugenie Aimée, Jiatsa Mbouna Cedric Derick, Tsouh Fokou Patrick Valere, Sahal Dinkar, Fekam Boyom Fabrice
Antimicrobial & Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé P.O. Box 812, Cameroon.
Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
Medicines (Basel). 2018 Oct 29;5(4):115. doi: 10.3390/medicines5040115.
In the midst of transient victories by way of insecticides against mosquitoes or drugs against malaria, the most serious form of malaria, caused by , continues to be a major public health problem. The emergence of drug-resistant malaria parasites facilitated by fake medications or the use of single drugs has worsened the situation, thereby emphasizing the need for a continued search for potent, safe, and affordable new antimalarial treatments. In line with this need, we have investigated the antiplasmodial activity of 66 different extracts prepared from 10 different medicinal plants that are native to Cameroon. Extracts were evaluated for their capacity to inhibit the growth of the chloroquine-sensitive (3D7) and resistant (INDO) strains of using the SYBR green fluorescence method. The cytotoxicity of promising extracts against human embryonic kidney cells (HEK293T) mammalian cells was assessed by MTT assay. The antiplasmodial activity (50% inhibitory concentration, IC) of plant extracts ranged from 1.90 to >100 μg/mL against the two strains. Six extracts exhibited good activity against both 3D7 and INDO strains, including cold water, water decoction, and ethyl acetate extracts of leaves of (Müll.Arg.) Hutch. (IC3D7/INDO = 4.91/6.64 μg/mL, 5.49/5.98 μg/mL, and 6.49/7.10 μg/mL respectively), water decoction extract of leaves of L. (IC3D7/INDO = 6.41/8.10 μg/mL), and water decoction extracts of leaves and bark of H.Perrier (IC3D7/INDO = 2.49/1.90 μg/mL and 3.70/2.80 μg/mL respectively). These promising extracts showed no cytotoxicity against HEK293T up to 200 μg/mL, giving selectivity indices (SIs) in the range of >31.20⁻80.32. While providing credence to the use of , , and in the traditional treatment of malaria, the results achieved set the stage for isolation and identification of active principles and ancillary molecules that may provide us with new drugs or drug combinations to fight against drug-resistant malaria.
在通过杀虫剂灭蚊或药物治疗疟疾（最严重的疟疾形式）取得短暂胜利的过程中，由[疟原虫种类未明确写出]引起的疟疾仍然是一个重大的公共卫生问题。假药或单一药物的使用导致耐药疟原虫的出现，使情况更加恶化，因此强调需要继续寻找有效、安全且可负担的新型抗疟治疗方法。基于这一需求，我们研究了从喀麦隆本土的10种不同药用植物中制备的66种不同提取物的抗疟原虫活性。使用SYBR绿色荧光法评估提取物抑制氯喹敏感株（3D7）和耐药株（INDO）[疟原虫种类未明确写出]生长的能力。通过MTT法评估有前景的提取物对人胚肾细胞（HEK293T）哺乳动物细胞的细胞毒性。植物提取物对两种菌株的抗疟原虫活性（50%抑制浓度，IC）范围为1.90至>100μg/mL。六种提取物对3D7和INDO菌株均表现出良好活性，包括[植物名称未明确写出]（Müll.Arg.）Hutch.叶片的冷水提取物、水煎剂提取物和乙酸乙酯提取物（IC3D7/INDO分别为4.91/6.64μg/mL、5.49/5.98μg/mL和6.49/7.10μg/mL）、L.叶片的水煎剂提取物（IC3D7/INDO = 6.41/8.10μg/mL）以及H.Perrier叶片和树皮的水煎剂提取物（IC3D7/INDO分别为2.49/1.90μg/mL和3.70/2.80μg/mL）。这些有前景的提取物在浓度高达200μg/mL时对HEK293T没有细胞毒性，选择性指数（SIs）在>31.20⁻80.32范围内。在为[植物名称未明确写出]、[植物名称未明确写出]和[植物名称未明确写出]在疟疾传统治疗中的应用提供可信度的同时，所取得的结果为分离和鉴定活性成分及辅助分子奠定了基础，这些成分可能为我们提供对抗耐药疟疾的新药或药物组合。
