Faculty of Science, University of Ngaoundere, B.P 454 Ngaoundere, Cameroon.
Parasitol Res. 2012 Aug;111(2):827-34. doi: 10.1007/s00436-012-2905-y. Epub 2012 Apr 5.
The aim of the study was to screen 11 selected traditional medicinal plants from West Africa for their in vitro antiplasmodial activity in order to determine the activity of single and of combination of plant extracts and to examine the activity of isolated pure compounds. Ethanolic and aqueous extracts of the 11 selected plants and pure compounds from Phyllanthus muellerianus and Anogeissus leiocarpus were tested in vitro against Plasmodium falciparum 3D7. Proliferation inhibitory effects were monitored after 48 h. Among the plants and pure compounds investigated in this study, geraniin from P. muellerianus, ellagic, gentisic, and gallic acids from A. leiocarpus, and extracts from A. leiocarpus, P. muellerianus and combination of A. leiocarpus with P. muellerianus affected the proliferation of P. falciparum most potently. Significant inhibitory activity was observed in combination of A. leiocarpus with P. muellerianus (IC(50) = 10.8 μg/ml), in combination of A. leiocarpus with Khaya senegalensis (IC(50) = 12.5 μg/ml), ellagic acid (IC(50) = 2.88 μM), and geraniin (IC(50) = 11.74 μM). In general growth inhibition was concentration-dependent revealing IC(50) values ranging between 10.8 and -40.1 μg/ml and 2.88 and 11.74 μM for plant extracts and pure substances respectively. Comparison with literature sources of in vivo and in vitro toxicity data revealed that thresholds are up to two times higher than the determined IC(50) values. Thus, the present study suggests that geraniin from P. muellerianus; ellagic acid, gallic acid, and gentisic acid from A. leiocarpus; and combination of extracts from A. leiocarpus with either P. muellerianus or K. senegalensis could be a potential option for malaria treatment.
这项研究的目的是筛选来自西非的 11 种传统药用植物，以检测其体外抗疟原虫活性，确定植物提取物的单一和组合活性，并研究分离出的纯化合物的活性。我们用乙醇和水提取 11 种选定植物的提取物和 Phyllanthus muellerianus 和 Anogeissus leiocarpus 的纯化合物，然后在体外检测它们对 Plasmodium falciparum 3D7 的抑制作用。48 小时后监测增殖抑制作用。在这项研究中，从 P. muellerianus 中提取的 geraniin、从 A. leiocarpus 中提取的 ellagic、gentisic 和 gallic 酸以及从 A. leiocarpus、P. muellerianus 和 A. leiocarpus 与 P. muellerianus 组合中提取的提取物对 P. falciparum 的增殖影响最为显著。A. leiocarpus 与 P. muellerianus 的组合（IC50=10.8 μg/ml）、A. leiocarpus 与 Khaya senegalensis 的组合（IC50=12.5 μg/ml）、ellagic 酸（IC50=2.88 μM）和 geraniin（IC50=11.74 μM）表现出显著的抑制活性。总的来说，生长抑制与浓度呈依赖性关系，植物提取物和纯物质的 IC50 值分别为 10.8-40.1 μg/ml 和 2.88-11.74 μM。与体内和体外毒性数据的文献来源比较表明，这些阈值比确定的 IC50 值高 2 倍。因此，本研究表明，P. muellerianus 中的 geraniin；A. leiocarpus 中的 ellagic acid、gallic acid 和 gentisic acid；以及 A. leiocarpus 提取物与 P. muellerianus 或 K. senegalensis 的组合，可能是治疗疟疾的一个潜在选择。
